• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Disease >New Isolates of Sweet potato feathery mottle virus and Sweet potato virus C: Biological and Molecular Properties, and Recombination Analysis Based on Complete Genomes
【24h】

New Isolates of Sweet potato feathery mottle virus and Sweet potato virus C: Biological and Molecular Properties, and Recombination Analysis Based on Complete Genomes

机译：红薯羽毛斑点病毒和甘薯病毒C的新分离株：生物和分子特性，以及基于完整基因组的重组分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sweet potato feathery mottle virus (SPFMV) and Sweet potato virus C (SPVC) isolates were obtained from sweetpotato shoot or tuberous root samples from three widely separated locations in Australia's tropical north (Cairns, Darwin, and Kununurra). The samples were planted in the glasshouse and scions obtained from the plants were graft inoculated to Ipomoea setosa plants. Virus symptoms were recorded in the field in Kununurra and in glasshouse-grown sweetpotato and I. setosa plants. RNA extracts from I. setosa leaf samples were subjected to high throughput sequencing. New complete SPFMV (n = 17) and SPVC (n = 6) genomic sequences were obtained and compared with 47 sequences from GenBank. Phylogenetic analysis revealed that the 17 new SPFMV genomes all fitted within either major phylogroup A, minor phylogroup II, formerly 0; or major phylogroup B, formerly RC. Major phylogroup A's minor phylogroup I, formerly EA, only appeared when recombinants were included. Numbers of SPVC genomes were insufficient to subdivide it into phylogroups. Within phylogroup A's minor phylogroup II, the closest genetic match between an Australian and a Southeast Asian SPFMV sequence was the 97.4% nucleotide identity with an East Timorese sequence. Recombination analysis of the 43 SPFMV and 27 SPVC sequences revealed evidence of 44 recombination events, 16 of which involved interspecies sequence transfers between SPFMV and SPVC and 28 intraspecies transfers, 17 in SPFMV and 11 in SPVC. Within SPFMV, 11 intraspecies recombination events were between different major phylogroups and 6 were between members of the same major phylogroup. Phylogenetic analysis accounting for the detected recombination events within SPFMV sequences yielded evidence of minor phylogroup II and phylogroup B but the five sequences from minor phylogroup I were distributed in two separate groups among the sequences of minor phylogroup II. For the SPVC sequences, phylogenetic analysis accounting for the detected recombination events revealed three major phylogroups (A, B, and C), with major phylogroup A being further subdivided into two minor phylogroups. Within the recombinant genomes of both viruses, their PI, NIa-Pro, NIb, and CP genes contained the highest numbers of recombination breakpoints. The high frequency of interspecies and interphylogroup recombination events reflects the widespread occurrence of mixed SPVC and SPFMV infections within sweetpotato plants. The prevalence of infection in northern Australian sweetpotato samples reinforces the need for improved virus testing in healthy sweetpotato stock programs. Furthermore, evidence of genetic connectivity between Australian and East Timorese SPFMV genomes emphasizes the need for improved biosecurity measures to protect against potentially damaging international virus movements.
机译：甘薯羽毛斑点病毒（SPFMV）和甘薯病毒C（SPVC）分离物是从澳大利亚热带北（凯恩斯，达尔文和Kununurra）的三个广泛分离的地点的甘薯芽或茎根样本。将样品种植在玻璃室中，从植物获得的间隙接种到IPOMOEA套管植物。病毒症状在Kununurra和Glasshouse-Slowlow SweetPotato和I. Setosa植物中记录在野间植物中。来自I. Setosa叶样品的RNA提取物经受高通量测序。获得新的完整SPFMV（n = 17）和SPVC（n = 6）基因组序列，并与来自GENBANK的47个序列进行比较。系统发育分析表明，17个新的SPFMV基因组均安装在主要的文学A，次要的植物群II中，以前0;或主要的Phylogroup B，以前是RC。主要的Phylogroup A的次要Phylogroup I，以前EA才出现在包含重组剂时。 SPVC基因组的数量不足以将其细分为phylogroups。在Phylogroup A的次要文学中II中，澳大利亚和东南亚SPFMV序列之间最近的遗传匹配是97.4％的核苷酸同一性与东帝汶序列。 43 SPFMV和27个SPVC序列的重组分析显示了44个重组事件的证据，其中16例涉及SPFMV和SPVC和28个内侧转移的序列转移，SPVC中的SPFMV和11中的17个。在SPFMV中，在不同主要的晶粒组中，11个内部重组事件在同一主要晶粒组的成员之间。 Phylocy分析SPFMV序列中检测到的重组事件核算产生的次要植物群II和植物群B的次数，但是从次次植物群II的序列中分布在两个单独的组中。对于SPVC序列，检测到的重组事件的系统发育分析算表现出三种主要的文学群（A，B和C），具有主要的晶类A进一步细分为两个次要的植物组。在病毒的重组基因组内，它们的PI，NIA-PRO，NIB和CP基因含有最多的重组断点。间隙的高频和侧髁类重组事件反映了甘薯植物中混合SPVC和SPFMV感染的广泛发生。澳大利亚北部感染的患病率甜美点样品增强了在健康的SweetPOTATO股票计划中改进病毒检测的需求。此外，澳大利亚和东帝汶SPFMV基因组之间的遗传连通性的证据强调了需要改进的生物安全措施，以防止可能损害国际病毒运动。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号