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首页> 外文期刊>Ultrasound in Medicine and Biology >Investigation Into the Impact of Diagnostic Ultrasound with Microbubbles on the Capillary Permeability of Rat Hepatomas
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Investigation Into the Impact of Diagnostic Ultrasound with Microbubbles on the Capillary Permeability of Rat Hepatomas

机译：微泡诊断超声对大鼠肝癌毛细血管渗透性的影响

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摘要

Ultrasound-targeted microbubble destruction (UTMD) takes advantage of transiently increased capillary permeability to enhance the release of tumor-specific drugs from blood vessels into sonicated tumor tissues. However, the application of focused ultrasound is limited because of the lack of an appropriate image-monitoring system. In this study, hepatoma-bearing Sprague-Dawley rats were insonicated with low-frequency diagnostic ultrasound and injected with Evans Blue (EB) dye and microbubbles through their tail veins to test changes in capillary permeability. We studied how the mechanical index, sonication duration and the injected microbubble (MB) concentration affect the hepatoma vascular permeability by quantitatively evaluating the EB delivery efficiency. Confocal laser scanning microscopy was used to observe the deposition of red fluorescence-dyed EB in tumor tissues. In addition, P-selectin, a type of biochemical marker that reflects vascular endothelial cell activation, was identified using an immunoblotting analysis. The experimental results reveal that EB delivery efficiency in tumor tissues was greater in groups with the diagnostic ultrasound-mediated UTMD (8.40 ± 0.71 %ID/g) than in groups without UTMD (1.73 ± 0.19 %ID/g) and EB delivery efficiency could be affected by MI, sonication duration and MB dose. The immunoblotting analysis indicates that diagnostic ultrasound-induced UTMD results in the vascular endothelial cell activation to increase capillary permeability, justifying the high quantity of EB deposited in tumor tissues.
机译：超声靶向微泡破坏（UTMD）利用瞬时提高的毛细血管渗透性，以增强从血管中释放肿瘤的药物到超声化的肿瘤组织中。然而，由于缺乏适当的图像监测系统，聚焦超声的应用是有限的。在这项研究中，肝脏瘤Sprague-Dawley大鼠用低频诊断超声波不透彻，并通过其尾静脉注射Evans Blue（EB）染料和微泡以测试毛细血管渗透性的变化。我们研究了如何通过定量评估EB输送效率来影响机械指数，超声检查持续时间和注射的微泡（MB）浓度影响肝癌血管渗透性。共聚焦激光扫描显微镜用于观察肿瘤组织中的红色荧光EB的沉积。此外，使用免疫印迹分析鉴定了反映血管内皮细胞活化的一种生化标记物的p-Selectin。实验结果表明，肿瘤组织中的EB递送效率与诊断超声介导的UTMD（8.40±0.71％ID / g）的组大于没有UTMD的组（1.73±0.19％ID / G）和EB交付效率受Mi，超声持续时间和Mb剂量的影响。免疫印迹分析表明，诊断超声诱导的UTMD导致血管内皮细胞活化以增加毛细血管渗透性，证明在肿瘤组织中沉积的高量EB。

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  • 来源
    《Ultrasound in Medicine and Biology》 |2013年第4期|共10页
  • 作者

    WangG.; ZhuoZ.; XiaH.; ZhangY.; HeY.; TanW.; GaoY.;

  • 作者单位

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

    Department of Ultrasound Xinqiao Hospital The Third Military Medical University Chongqing China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 影像诊断学;
  • 关键词

    Blood-tumor barrier; Capillary permeability; Diagnostic ultrasound; Evans blue; Microbubbles;

    机译：血肿瘤屏障;毛细血管渗透性;诊断超声;埃文斯蓝;微泡;

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