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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Use of qpcr and genomic sequencing to diagnose plasmodium ovale wallikeri malaria in a returned soldier in the setting of a negative rapid diagnostic assay
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Use of qpcr and genomic sequencing to diagnose plasmodium ovale wallikeri malaria in a returned soldier in the setting of a negative rapid diagnostic assay

机译：使用QPCR和基因组测序在返回士兵中诊断疟原虫霍米利湖疟疾，在返回的士兵中进行负迅速诊断测定

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摘要

Plasmodium ovale is one of several clinically relevant malaria species known to cause disease in humans. However, in contrast to Plasmodium falciparum and Plasmodium vivax, which are responsible for most cases of human malaria, P. ovale has a wide distribution but low prevalence in tropical regions. Here, we report the case of a soldier returning from Liberia with P. ovale wallikeri malaria. This case highlights the limitations of both microscopy and the malaria rapid diagnostic test for diagnosing infection with P. ovale and for distinguishing P. ovale wallikeri from P. ovale curtisi. To our knowledge, this is the first case report in which quantitative real-time polymerase chain reaction amplification using the Cytochrome B gene, coupled with genomic sequencing of the potra locus, was used for definitive diagnosis of P. ovale wallikeri malaria.
机译：疟原虫卵石是众所周知的临床相关的疟疾物种之一，称为人类疾病。 然而，与疟原虫和疟原虫疟原虫疟原虫，这是对大多数人疟疾病例的疟原虫，P.卵巢具有广泛的分布，但热带地区的普遍存在。 在这里，我们举报了利比里亚与P. Ovale Wallikeri Malaria返回的士兵的案件。 这种情况突出了显微镜的局限性和疟疾快速诊断测试，用于诊断P.卵形卵巢感染，并从P. Overal Curtisi区分P. Ovale Wallikeri。 据我们所知，这是第一种案例报告，其中使用细胞色素B基因的定量实时聚合酶链反应扩增与POTRA基因座的基因组测序相结合，用于明确的P. Ovale Wallikeri疟疾诊断。

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    CohenR.; FeghaliK.; AlemayehuS.; KomisarJ.; HangJ.; WeinaP.J.; CoggeshallP.; KamauE.; ZaporM.;

  • 作者单位

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

    Infectious Disease Service Walter Reed National Military Medical Center 8901 Wisconsin Avenue;

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  • 正文语种 eng
  • 中图分类 地方病学;
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