...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Astrocyte-restricted disruption of connexin-43 impairs neuronal plasticity in mouse barrel cortex
【24h】

Astrocyte-restricted disruption of connexin-43 impairs neuronal plasticity in mouse barrel cortex

机译：星形胶质细胞限制Connexin-43的破坏损害小鼠桶皮层中的神经元可塑性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is intensive gap-junctional coupling between glial processes, but their significance in sensory functions remains unknown. Connexin-43 (Cx43), a major component of astrocytic gap-junction channels, is abundantly expressed in astrocytes. To investigate the role of Cx43-mediated gap junctions between astrocytes in sensory functions, we generated Cx43 knockout (KO) mice with a mouse line carrying loxP sites flanking exon 2 of the Cx43 gene and the transgenic line expressing Cre recombinase under control of the glial fibrillary acidic protein promoter, which exhibited a significant loss of Cx43 in astrocytes in the barrel cortex. Although Cx43 expression between the astrocytes measured by immunohistochemistry was virtually abolished in Cx43 KO mice, they had normal architecture in the barrel cortex but the intensity of cytochrome oxide histochemistry decreased significantly. In vivo electrophysiological analysis revealed that the long-term potentiation of the vibrissal evoked responses in the barrel cortex evoked by high-frequency rhythmic vibrissal stimuli (100 Hz, 1 s) was abolished in Cx43 KO mice. Current source density analysis also revealed that astrocytic Cx43 was important to the flow of excitation within the laminar connections in barrel cortex. Behavioral tests showed that the ability of Cx43 KO mice to sense the environment with their whiskers decreased. Even so, the jump-stand experiment showed that they could still discriminate rough from smooth surfaces. Our findings suggest that Cx43-mediated gap-junctional coupling between astrocytes is important in the neuron-glia interactions required for whisker-related sensory functions and plasticity.
机译：胶质过程之间存在密集的差距和耦合，但它们在感官功能中的意义仍然未知。 Connexin-43（CX43）是星形胶质型间隙结沟道的主要成分，在星形胶质细胞中大量表达。为了研究CX43介导的间隙连接在感觉函数中的星形胶质细胞之间的作用，我们用CX43基因的侧翼外显子2的小鼠线的CX43敲除（KO）小鼠和在胶质的控制下表达CRE重组酶的转基因系列纤维状酸性蛋白促进剂，其在桶皮层中的星形胶质细胞中表现出显着的CX43。虽然通过免疫组织化学测量的星形胶质细胞之间的CX43表达在CX43 KO小鼠中几乎被废除，但它们在桶皮层中具有正常的结构，但细胞色素氧化物组织化学的强度显着下降。在体内电生理学分析表明，在CX43 KO小鼠中废除了通过高频节律vibrissal刺激（100Hz，1 s）引起的桶皮层中颤动诱发反应的长期增强。电流源密度分析还显示星形胶质细胞CX43对桶皮层中的层状连接内的激发流动是重要的。行为试验表明，CX43 KO小鼠的能力感觉与晶须的环境减少。即便如此，跳台实验表明它们仍然可以从光滑的表面区分粗糙度。我们的研究结果表明，星形胶质细胞之间的CX43介导的间隙结合在晶须相关的感官功能和可塑性所需的神经元 - 胶质胶质相互作用中是重要的。

著录项

  • 来源
  • 作者

    HanY.; YuH.-X.; SunM.-L.; WangY.; XiW.; YuY.-Q.;

  • 作者单位

    Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang Province Key;

    Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang Province Key;

    Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang Province Key;

    Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang Province Key;

    Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang Province Key;

    Key Laboratory of Medical Neurobiology of the Ministry of Health of China Zhejiang Province Key;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 神经病学与精神病学;
  • 关键词

    Behavior; Conditional knockout; Cre/loxP; Current source density; Long-term potentiation;

    机译：行为;条件淘汰;CRE / LOXP;电流源密度;长期倾销;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号