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首页> 外文期刊>The European Journal of Neuroscience >Periodic properties of the histaminergic system of the mouse brain
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Periodic properties of the histaminergic system of the mouse brain

机译：小鼠脑中组胺能系统的周期性特性

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Brain histamine is involved in the regulation of the sleep-wake cycle and alertness. Despite the widespread use of the mouse as an experimental model, the periodic properties of major markers of the mouse histaminergic system have not been comprehensively characterized. We analysed the daily levels of histamine and its first metabolite, 1-methylhistamine, in different brain structures of C57BL/6J and CBA/J mouse strains, and the mRNA level and activity of histidine decarboxylase and histamine-N-methyltransferase in C57BL/6J mice. In the C57BL/6J strain, histamine release, assessed by in vivo microdialysis, underwent prominent periodic changes. The main period was 24 h peaking during the activity period. Additional 8 h periods were also observed. The release was highly positively correlated with active wakefulness, as shown by electroencephalography. In both mouse strains, tissue histamine levels remained steady for 24 h in all structures except for the hypothalamus of CBA/J mice, where 24-h periodicity was observed. Brain tissue 1-methylhistamine levels in both strains reached their maxima in the periods of activity. The mRNA level of histidine decarboxylase in the tuberomamillary nucleus and the activities of histidine decarboxylase and histamine-N-methyltransferase in the striatum and cortex did not show a 24-h rhythm, whereas in the hypothalamus the activities of both enzymes had a 12-h periodicity. These results show that the activities of histamine-metabolizing enzymes are not under simple direct circadian regulation. The complex and non-uniform temporal patterns of the histaminergic system of the mouse brain suggest that histamine is strongly involved in the maintenance of active wakefulness.
机译：脑组胺涉及睡眠唤醒循环和警觉性的调节。尽管鼠标广泛使用作为实验模型，但小鼠组蛋白能系统的主要标志物的周期性尚未全面表征。我们分析了C57BL / 6J和CBA / J小鼠菌株的不同脑结构中组胺及其第一次代谢物，1-甲基胺，以及组氨酸脱羧酶和组胺-N-甲基转移酶的mRNA水平和活性的第一种代谢物。老鼠。在C57BL / 6J菌株中，组胺释放，通过体内微透析评估，进行了突出的周期性变化。在活动期间，主要时期为24小时达到尖峰。还观察到额外的8小时。如脑电图所示，释放与主动助出性高度相关。在两个小鼠菌株中，除了CBA / J小鼠的下丘脑中，组织组胺水平在所有结构中持续24小时，其中观察到24小时周期性。两种菌株中的脑组织1-甲基萘胺水平在活动期间达到了最大值。结核菌核中的组氨酸脱羧酶的mRNA水平和纹状体中的组氨酸脱羧酶和组胺-N-甲基转移酶的活性并未显示出24-h节奏，而在下丘脑中，两种酶的活动有12-h周期性。这些结果表明，组胺代谢酶的活性不是在简单的直接昼夜调节下。小鼠脑的组蛋白能系统的复合物和不均匀的时间模式表明组胺强烈地参与维持活性醒来。

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