...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Distinct subsynaptic localization of type 1 metabotropic glutamate receptors at glutamatergic and GABAergic synapses in the rodent cerebellar cortex
【24h】

Distinct subsynaptic localization of type 1 metabotropic glutamate receptors at glutamatergic and GABAergic synapses in the rodent cerebellar cortex

机译：1型代谢谷氨酸受体在谷氨酸和啮齿动物皮层中的三种代购虫谷氨酸受体的不同休税局部化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Type 1 metabotropic glutamate (mGlu1) receptors play a pivotal role in different forms of synaptic plasticity in the cerebellar cortex, e.g. long-term depression at glutamatergic synapses and rebound potentiation at GABAergic synapses. These various forms of plasticity might depend on the subsynaptic arrangement of the receptor in Purkinje cells that can be regulated by protein-protein interactions. This study investigated, by means of the freeze-fracture replica immunogold labelling method, the subcellular localization of mGlu1 receptors in the rodent cerebellum and whether Homer proteins regulate their subsynaptic distribution. We observed a widespread extrasynaptic localization of mGlu1 receptors and confirmed their peri-synaptic enrichment at glutamatergic synapses. Conversely, we detected mGlu1 receptors within the main body of GABAergic synapses onto Purkinje cell dendrites. Although Homer proteins are known to interact with the mGlu1 receptor C-terminus, we could not detect Homer3, the most abundant Homer protein in the cerebellar cortex, at GABAergic synapses by pre-embedding and post-embedding immunoelectron microscopy. We then hypothesized a critical role for Homer proteins in the peri-junctional localization of mGlu1 receptors at glutamatergic synapses. To disrupt Homer-associated protein complexes, mice were tail-vein injected with the membrane-permeable dominant-negative TAT-Homer1a. Freeze-fracture replica immunogold labelling analysis showed no significant alteration in the mGlu1 receptor distribution pattern at parallel fibre-Purkinje cell synapses, suggesting that other scaffolding proteins are involved in the peri-synaptic confinement. The identification of interactors that regulate the subsynaptic localization of the mGlu1 receptor at neurochemically distinct synapses may offer new insight into its trafficking and intracellular signalling.
机译：1型代谢谷氨酸（MGLU1）受体以小脑皮质中的不同形式的突触塑性发挥枢轴作用，例如，在Gabaergic突触的谷氨酸盐酸胶囊突触和反弹抑制的长期抑郁症。这些各种形式的可塑性可能取决于Purkinje细胞中受体的副腹膜排列，其可以通过蛋白质 - 蛋白质相互作用调节。本研究通过冷冻骨折复制物免疫标记方法研究，啮齿动物小脑中MGLU1受体的亚细胞定位以及HOMER蛋白是否调节其抗腹腹部分布。我们观察到MGLU1受体的广泛促进突触局部化，并确认了它们在谷氨酰胺突触处的Peri-Synaptic富集。相反，我们发现了Gabaergic突触主体内的MglU1受体在Purkinje Cell Dendrites上。虽然已知HOMER蛋白与MGLU1受体C-末端相互作用，但是通过预嵌入和嵌入免疫电解显微镜，我们无法在GABAGERIC突触处检测HOMER3。然后，假设Homer蛋白在谷氨酸突突突突中MGLU1受体的PERI-结定位中的主要作用。为了破坏荷马相关的蛋白质复合物，小鼠尾静脉注射膜可渗透的优势阴性TAT-Homer1a。冻结复制品免疫偶极标记分析显示平行纤维 - 紫癜细胞突触的MGLU1受体分布模式无显着改变，表明其他支架蛋白涉及PERI-突触禁闭。调节神经化学突出突触在神经化学突出的突触中调节MGLU1受体的次腹定位的交流剂的鉴定可以提供新的洞察力和细胞内信号传导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号