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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Recent advances of Diels-Alderases involved in natural product biosynthesis
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Recent advances of Diels-Alderases involved in natural product biosynthesis

机译：DIELS- alderases的最新进展在天然产品生物合成中

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摘要

Frequent occurrence of [4+2] adducts in the secondary metabolites suggested involvement of Diels-Alderases (DAases) in their biosynthesis. However, a limited number of DAases were reported before early 2000s. Advancements in whole-genome sequencing and searching tool of the biosynthetic gene clusters of the secondary metabolites facilitate the identification of plausible DAases. Thus, during past 5 years, nine DAases have been characterized by genetic and biochemical analyses. These include a detailed functional analysis of SpnF that solely catalyzes [4+2] cycloaddition, a structural analysis of spirotetramate-forming enzyme PyrI4 complexed with the corresponding cycloadduct, and DAases catalyzing decalin formation and macrocyclic pyridine formation. Together with decalin-forming enzymes and macrocyclic pyridine-forming enzymes, these results provided sufficient data to discuss catalytic mechanism of DAases and nature's strategy for molecular diversification of linear chain intermediates derived from polyketide and ribosomal peptide biosynthetic machinery.
机译：次级代谢产物中频繁发生[4 + 2]加合物建议在其生物合成中的Diels- alderases（Daase）的参与。然而，在2000年代初期之前报告了有限数量的衰减。次级代谢物的生物合成基因簇的全基因组测序和搜索工具的进步促进了合理的叶片的鉴定。因此，在过去的5年中，遗传和生物化学分析的特征在于九个衰变。这些包括SPNF的详细功能分析，所述SPNF仅催化[4 + 2]环加入，螺旋体形成酶Pyri4与相应环形加成络合的结构分析，并达到催化癸蛋白形成和大环吡啶形成。这些结果与癸蛋白 - 形成酶和大环吡啶形成酶一起提供了足够的数据，以讨论衍生自聚酮和核糖体肽生物合成机械的线性链中间体分子多样化的催化机制和自然的策略。

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