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首页> 外文期刊>Biotechnology Progress >Immobilized yeast bioreactor systems for continuous beer fermentation
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Immobilized yeast bioreactor systems for continuous beer fermentation

机译：用于啤酒连续发酵的固定化酵母生物反应器系统

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摘要

Two different types of immobilized yeast bioreactors were examined for continuous fermentation of high-gravity worts. One of these is a fluidized bed reactor (FBR) that employs porous glass beads for yeast immobilization. The second system is a loop reactor containing a porous silicon carbide cartridge (SCCR) for immobilizing the yeast cells. Although there was some residual fermentable sugar in the SCCR system product, nearly complete attenuation of the wort sugars was achieved in either of the systems when operated as a two-stage process. Fermentation could be completed in these systems in only half the time required for a conventional batch process. Both the systems showed similar kinetics of extract consumption, and therefore similar volumetric productivity. As compared to the batch fermentation, total fusel alcohols were lower; total esters, while variable, were generally higher. The yeast biomass production was similar to that in a conventional fermentation process. As would be expected in an accelerated fermentation system, the levels of vicinal diketones (VDKs) were higher. To remove the VDKs, the young beer was heat-treated to convert the VDK precursors and processed through a packed bed immobilized yeast bioreactor for VDK assimilation. The finished product from the FBR system was found to be quite acceptable from a flavor perspective, albeit different from the product from a conventional batch process. Significantly shortened fermentation times demonstrate the feasibility of this technology for beer production.
机译：检查了两种不同类型的固定化酵母生物反应器，以进行高重力麦芽汁的连续发酵。其中之一是使用多孔玻璃珠固定酵母的流化床反应器（FBR）。第二系统是回路反应器，其包含用于固定酵母细胞的多孔碳化硅药筒（SCCR）。尽管SCCR系统产品中有一些残留的可发酵糖，但是当以两步法运行时，在任何一个系统中麦汁糖几乎都能完全衰减。在这些系统中，发酵只需完成常规分批过程所需时间的一半即可。两种系统均显示出相似的萃取物消耗动力学，因此具有相似的体积生产率。与分批发酵相比，杂醇的总含量较低。总酯虽然可变，但通常较高。酵母生物量的生产与常规发酵过程中的相似。正如加速发酵系统中预期的那样，邻位二酮（VDK）的含量较高。为了除去VDK，将年轻啤酒进行热处理以转化VDK前体，并通过固定床固定的酵母生物反应器进行处理，以进行VDK同化。从FBR系统得到的成品从风味角度来看是可以接受的，尽管与常规分批工艺的产品有所不同。大大缩短的发酵时间证明了该技术在啤酒生产中的可行性。

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