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首页> 外文期刊>Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research >Immunohistochemistry of aberrant neuronal development induced by 6-propyl-2-thiouracil in rats
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Immunohistochemistry of aberrant neuronal development induced by 6-propyl-2-thiouracil in rats

机译：6-丙基-2-硫酸中的异常神经元发育的免疫组化学

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摘要

6-Propyl-2-thiouracil (PTU)-induced hypothyroidism disrupts neuronal/glial development. This study sought to identify the sensitive immunohistochemical parameters of developmental neurotoxicity (DNT) following PTU-exposure, as well as their responses in a 28-day toxicity study in adults. In the developmental exposure study, pregnant rats were treated with 0, 1, 3, and 10 ppm PTU in drinking water from gestational day 6 to postnatal day (PND) 21 and pups were examined on PNDs 21 and 77. In the adult-stage exposure study, 5-week-old male rats were treated with 0, 0.1 and 10 mg PTU/kg by oral gavage for 28 days. In the developmental exposure study on PND 21, there were fewer GFAP(+), PAX6(+), and DCX+ cells in the subgranular zone (SGZ) of the hippocampal dentate gyrus at >= 3 or 10 ppm. Regarding synaptic plasticity-related molecules, there were fewer EPHA4(+) and ARC(+) cells in the dentate granule cell layer. Regarding GABAergic interneuron subpopulations, there were more RELN+, CALB2(+), and SST+ cells and fewer PVALB(+) cells in the dentate hilus. There were also differences in the numbers of RELN+, PVALB(+), CALB2(+), and NPY+ cells in the cerebral cortex, and RELN+, PVALB(+), and SST+ cells in the cerebellar cortex. Most of these changes were sustained until PND 77. Following adult-stage exposure (10 mg/kg), there were fewer SGZ DCX+ cells, but more RELN+ and SST+ cells in the dentate hilus. Results suggest that GABAergic interneuron populations in cortical tissues, hippocampal neurogenesis, and synaptic plasticity are sensitive to PTU-induced DNT during development. In contrast, only hippocampal neurogenesis was sensitive to adult-stage exposure. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
机译：6丙基-2-硫嘧啶（PTU） - 诱导的甲状腺功能亢进破坏了神经元/胶质发育。该研究寻求鉴定PTU暴露后发育神经毒性（DNT）的敏感免疫组织化学参数，以及其在成人28天毒性研究中的反应。在发育曝光研究中，将孕大鼠用0,1,3和10ppm PTU治疗，从妊娠期6天饮用水中的饮用水中的第6天（PND）21，在PNDS 21和77上检查幼崽。在成人阶段暴露研究，5周龄雄性大鼠用0,0.1和10mg PTU / kg进行口服饲养28天。在对PND 21的发育曝光研究中，海马齿状齿状物谱系（SGZ）中的GFAP（+），PAX6（+）和DCX +细胞较少，在> = 3或10ppm处。关于突触塑性相关的分子，牙齿颗粒细胞层中的EphA4（+）和弧（+）细胞较少。关于加布性的中性亚群，在牙齿Hilus中还有更多的Reln +，Calb2（+）和SST +细胞和更少的PVALB（+）细胞。在大脑皮层中的Reln +，PVALB（+），CALB2（+）和NPY +细胞的数量也存在差异，并且在小脑皮质中的CRERN +，PVALB（+）和SST +细胞中。这些变化中的大部分是持续的，直到PND 77.在成人阶段暴露（10mg / kg）之后，Sgz DCx +细胞较少，但牙齿Hilus中的更多Reln +和SST +细胞。结果表明，在皮质组织，海马神经发生和突触塑性中的胃肠杆菌性脑中群体对开发过程中的PTU诱导的DNT敏感。相比之下，只有海马神经发生对成人阶段暴露敏感。 （c）2016 Elsevier Ireland Ltd.保留所有权利。

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