Development of real-time PCR assay for the detection of Mycoplasma bovis

Behera S.; Rana R.; Gupta P. K.; Kumar D.; Sonal; Rekha V.; Arun T. R.; Jena D.

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Development of real-time PCR assay for the detection of Mycoplasma bovis

机译：用于检测支原体BOVI的实时PCR测定的发展

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Mycoplasma bovis is one of the important bovine mycoplasma involved in economically important clinical conditions like respiratory diseases, otitis media, and mastitis. The present study was undertaken with the objective of developing a SYBR Green dye-based real-time PCR assay targeting uvrC gene for the diagnosis of M. bovis. The analytical sensitivity and specificity of the assay were evaluated. The test showed 10(3)-fold more sensitivity than conventional PCR and detected down to 100 fg level of DNA. It was found to be specific, as no cross reactivity was shown with other related bacteria and Mycoplasma species. The developed assay was able to detect down to 40 copies of uvrC gene from spiked bovine milk samples. At present, this developed assay may be used as a valuable diagnostic tool for the detection of Mycoplasma bovis.

机译：支原体Bovis是伴随着呼吸系统疾病，中耳炎和乳腺炎等经济上重要的临床病症的重要牛支原体之一。本研究采取目的是开发SYBR绿色染料的实时PCR测定靶向UVRC基因，用于诊断M. Bovis。评估测定的分析敏感性和特异性。该测试显示10（3） - 比常规PCR更多的灵敏度，并检测到100℃的DNA水平。发现它是特异性的，因为没有用其他相关细菌和支原体物种显示交叉反应性。开发的测定能够从尖刺牛奶样品中检测到40份UVRC基因。目前，该开发的测定可以用作检测支原体Bovis的有价值的诊断工具。

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来源
《Tropical Animal Health and Production》 |2018年第4期|共8页
作者
Behera S.; Rana R.; Gupta P. K.; Kumar D.; Sonal; Rekha V.; Arun T. R.; Jena D.;
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作者单位

IVRI Div Bacteriol &

Mycol Bareilly Uttar Pradesh India;

IVRI Div Bacteriol &

Mycol Bareilly Uttar Pradesh India;

IVRI Div Anim Biotechnol Bareilly Uttar Pradesh India;

IVRI Div Anim Biotechnol Bareilly Uttar Pradesh India;

IVRI Div Anim Biotechnol Bareilly Uttar Pradesh India;

IVRI Div Bacteriol &

Mycol Bareilly Uttar Pradesh India;

IVRI Div Bacteriol &

Mycol Bareilly Uttar Pradesh India;

IVRI Div Anim Reprod Bareilly Uttar Pradesh India;

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原文格式 PDF
正文语种 eng
中图分类畜牧、动物医学、狩猎、蚕、蜂;
关键词
Mycoplasma bovis; uvrC gene; Real-time PCR;

机译：支原体BOVI;UVRC基因;实时PCR;

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1. Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay ‡ [J] . Alesia Reinisch, Daniela Bruno, Debabrata Mahapatra, Veterinary Sciences . 2015,第1期

机译：快速检测牛支原体的新型Taqman实时PCR检测方法的开发和评估：与常规PCR检测方法和另一种Taqman实时PCR检测方法的检测性能比较
2. Development and Evaluation of a Novel Single-Nucleotide-Polymorphism Real-Time PCR Assay for Rapid Detection of Fluoroquinolone-Resistant Mycoplasma bovis [J] . M. Ben Shabat, I. Mikula, I. Gerchman, Journal of Clinical Microbiology . 2010,第8期

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3. Development of a duplex real-time TaqMan PCR assay with an internal control for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from commercial and backyard poultry [J] . Sprygin A. V., Andreychuk D. B., Kolotilov A. N., Avian Pathology . 2010,第2期

机译：开发带有内部对照的双重实时TaqMan PCR检测试剂盒，用于检测商业和后院家禽临床样品中的鸡支原体和滑膜支原体
4. DEVELOPMENT OF DNA EXTRACTION AND PCR AMPLIFICATION PROTOCOLS FOR DETECTION OF MYCOPLASMA BOVIS [C] . Paola Cremonesi, Chiara Vimercati, Gigliola Perez, Annual Meeting of the National Mastitis Council . 2006

机译：DNA提取和PCR扩增方案检测的Mycoplasma Bovis的开发
5. Development of a real-time PCR assay for detection and quantification of Escherichia coli O157:H7 in apple juice. [D] . DeTrana, Nancy Rabalais. 2007

机译：开发用于检测和定量苹果汁中大肠杆菌O157：H7的实时PCR检测方法。
6. Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay [O] . Hemant Naikare, Daniela Bruno, Debabrata Mahapatra, 2015

机译：快速检测牛支原体的新型Taqman实时PCR检测方法的开发和评估：与常规PCR检测方法和另一种Taqman实时PCR检测方法的检测性能比较
7. Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay [O] . Hemant Naikare, Daniela Bruno, Debabrata Mahapatra, 2015

机译：用于快速检测牛支原体的新型Taqman实时pCR检测方法的开发和评价：使用常规pCR检测和另一种Taqman实时pCR检测的检测性能比较

Development of real-time PCR assay for the detection of Mycoplasma bovis

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