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首页> 外文期刊>Digestive Diseases and Sciences >Does mutation of hepatitis A virus exist in North India?
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Does mutation of hepatitis A virus exist in North India?

机译：北印度是否存在甲型肝炎的突变？

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BACKGROUND: Human hepatitis A, a widespread infectious disease that is hyperendemic in vast areas of the world, results in the infection of the liver. Different human HAV strains of diverse geographic origin are remarkably closely related. HAV exploits all known mechanisms of genetic variation to ensure survival, including mutation and genetic recombination. OBJECTIVES: The aim of the study was to undertake an in-depth analysis of the mutation in three groups: (i) mild acute hepatitis (m-AH), (ii) severe acute hepatitis (s-AH), and (iii) fulminant hepatitis (FHF) A patients, who were tested positive for HAV RNA. MATERIALS AND METHODS: A total of 500 patients of acute viral hepatitis (AVH) were screened for HAV-IgM positivity from January 2003 to December 2004. HAV RNA positivity was subject to reverse transcription of RNA followed by polymerase chain reaction (RT-PCR) for the detection of HAV RNA. The HAV RNA positive cases were subject to single-stranded conformational polymorphism (SSCP). RESULTS: Out of 500 acute cases of hepatitis, 80 (16%) were positive for HAV-IgM. HAV RNA was detected in 34 (42.5%) cases by RT-PCR. Twenty-four (70.5%) were m-AH, seven (20.5%) were s-AH, and three (8.8%) were FHF. All the positive samples were subject to SSCP. No mobility shift was observed with respect to any screened samples by PCR-SSCP. Four (m-AHI-54, m-AHI-80, s-AHI-341 and FHFI-195 suspected cases were directly sequenced to prove that there was no point mutation. CONCLUSION: SSCP demonstrates no mobility shift in the VP1/P2A region of the HAV genome. No point mutation was observed in the four suspected cases by sequencing. However a large study from different geographical locations is needed to achieve a logical conclusion about the existence of HAV mutation in the Indian population.
机译：背景：人类甲型肝炎，一种广阔的传染病，在世界广阔地区，导致肝脏感染。不同的人物的不同地理来源的菌株非常相关。 HAV利用遗传变异的所有已知机制，以确保存活，包括突变和遗传重组。目的：该研究的目的是对三组的突变进行深入分析：（i）轻度急性肝炎（M-AH），（ii）严重急性肝炎（S-AH）和（iii）暴发性肝炎（FHF）患者，患者被测试为HAV RNA。材料和方法：2003年1月至2004年12月，筛选了500例急性病毒性肝炎（AVH）患者.HAV RNA阳性对RNA的逆转录，然后聚合酶链反应（RT-PCR）受到逆转录用于检测HAV RNA。 HAV RNA阳性病例受单链构象多态性（SSCP）。结果：500分急性肝炎病例，80例（16％）对HAV-IgM呈阳性。通过RT-PCR在34例（42.5％）病例中检测到HAV RNA。二十四（70.5％）为M-AH，七（20.5％）为S-AH，三（8.8％）为FHF。所有阳性样品都受SSCP。对于PCR-SSCP的任何筛选样品，不观察到迁移率。四（M-AHI-54，M-AHI-80，S-AHI-341和FHFI-195疑似病例被直接测序，以证明没有点突变。结论：SSCP在VP1 / P2A区域中展示了移动性移位HAV基因组。通过测序在四种可疑病例中观察到任何点突变。然而，需要从不同地理位置的大型研究来实现印度人群体存在于HAV突变的逻辑结论。

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