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首页> 外文期刊>Biotechnology Progress >Production of Phytase by Mucor racemosus in Solid-State Fermentation
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Production of Phytase by Mucor racemosus in Solid-State Fermentation

机译：固态发酵中的Mucor racemosus生产植酸酶

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摘要

Phytase production was studied bgy three Mucor and eitht Rhizopus strains by solid-state fermentation (SSF) on three commonly used natural feed ingredients (canola meal,coconut oil cake wheat bran).Mucor racemosus NRRL 1994 (ATCC 46129) gave the highest yield (14.5IU/g dry matter phytase activity)on coconut oil cake.Optimizing the supplementation of coconut oil cake with glucose casein and (NH_4)SO_4 phytase production in solid-state fermentation was increased to 26 IU/g dry matter (DM).Optimization was carried out by Plackett-Burman asnd central composite experimental designs.Using the optimized medium phytase, alpha-amylase and lipase production of Mucor racemosus NRRL 1994 was comjpared in solid-state fermentation and in shake flaks (SF) ferment ation .SSF yielded higher phytase activity than did SF based on mass of initial substrate.Because this particular isolate is a food-grade fungus that has been used for sufu fermentation in China,the whole SSF material (crude enzyme,in situ enzyme) may be used directly in amimal feed rations with 3enhanced cost efficiency.
机译：在三种常用的天然饲料原料（低芥酸菜粕，椰子油饼麦麸）上通过固态发酵（SSF）研究了三株Mucor和Rhizopus菌株的植酸酶生产。总状花序NRRL 1994（ATCC 46129）的产量最高（在椰子油饼上的14.5IU / g干物质植酸酶活性。优化在固态发酵中补充酪蛋白的葡萄糖和（NH_4）SO_4植酸酶的产量增加到26 IU / g干物质（DM）。由Plackett-Burman进行中央复合实验设计。使用优化的培养基植酸酶，固相发酵和摇瓶发酵（SF）发酵，可得到Mucor racemosus NRRL 1994的α-淀粉酶和脂肪酶。植酸酶的活性要比SF高，这是基于初始底物的质量。因为这种特殊的分离物是一种食品级真菌，已在中国用于sufu发酵，所以整个SSF物质（粗酶，原位酶（nzyme）可直接用于动物饲料中，提高了成本效益。

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