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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Enhanced apoptotic effects of dihydroartemisinin-aggregated gelatin and hyaluronan nanoparticles on human lung cancer cells
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Enhanced apoptotic effects of dihydroartemisinin-aggregated gelatin and hyaluronan nanoparticles on human lung cancer cells

机译：增强二氢氨苄蛋白聚集的明胶和透明质酸纳米粒子对人肺癌细胞的凋亡作用

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摘要

Recent studies suggest that dihydroartemisinin (DHA), a derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua L., has anticancer properties. Due to poor water solubility, poor oral activity, and a short plasma half-life, large doses of DHA have to be injected to achieve the necessary bioavailability. This study examined increasing DHA bioavailability by encapsulating DHA within gelatin (GEL) or hyaluronan (HA) nanoparticles via an electrostatic field system. Observations from transmission electron microscopy show that DHA in GEL and HA nanoparticles formed GEL/DHA and HA/DHA aggregates that were approximately 30-40 nm in diameter. The entrapment efficiencies for DHA were approximately 13 and 35% for the GEL/DHA and HA/DHA aggregates, respectively. The proliferation of A549 cells was inhibited by the GEL/DHA and HA/DHA aggregates. Fluorescent annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining displayed low background staining with annexin V-FITC or PI on DHA-untreated cells. In contrast, annexin V-FITC and PI stains dramatically increased when the cells were incubated with GEL/DHA and HA/DHA aggregates. These results suggest that DHA-aggregated GEL and HA nanoparticles exhibit higher anticancer proliferation activities than DHA alone in A549 cells most likely due to the greater aqueous dispersion after hydrophilic GEL or HA nanoparticles aggregation. These results demonstrate that DHA can aggregate with nanoparticles in an electrostatic field environment to form DHA nanosized aggregates.
机译：最近的研究表明，二氢甲醛（DHA），来自传统中草药Arremisia Annua L.中分离的青蒿素的衍生物，具有抗癌性质。由于水溶解度差，口腔活动差，血浆半衰期短，必须注射大剂量的DHA以达到必要的生物利用度。该研究通过静电场系统将DHA包封在明胶（凝胶）或透明质酸（HA）纳米颗粒中通过静电场系统检查了DHA生物利用度。来自透射电子显微镜的观察结果显示凝胶和HA纳米颗粒中的DHA形成凝胶/ DHA和HA / DHA聚集体，其直径约为30-40nm。凝胶/ DHA和HA / DHA骨料的DHA血管血管效率分别为约13和35％。凝胶/ DHA和HA / DHA聚集体抑制A549细胞的增殖。荧光膜蛋白V-荧光素异硫氰酸酯（FITC）和碘化丙锭（PI）染色显示在DHA-未处理的细胞上用膜蛋白V-FITC或PI染色的低背景。相反，当将细胞与凝胶/ DHA和HA / DHA聚集体一起温育时，膜蛋白V-FITC和PI污渍显着增加。这些结果表明，DHA聚集的凝胶和HA纳米颗粒在最有可能由于亲水性凝胶或HA纳米粒子聚集后的较大水分散体而具有更高的抗癌增殖活性。这些结果表明，DHA可以通过静电场环境中的纳米颗粒聚集，形成DHA纳米粒化聚集体。

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