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首页> 外文期刊>Journal of clinical laboratory analysis. >Polymorphisms of human leukocyte antigen B*27 on clinical phenotype of spondyloarthritis in Chinese
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Polymorphisms of human leukocyte antigen B*27 on clinical phenotype of spondyloarthritis in Chinese

机译：人白细胞抗原B * 27对脊椎关节炎临床表型的多态性

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Background In recent years, an ever‐increasing number of alleles of human leukocyte antigen B*27 ( HLA ‐B*27) have been identified. This study aimed to establish an updated method for HLA ‐B*27 subtyping, and to investigate the impact of HLA ‐B*27 polymorphisms on the clinical phenotype of spondyloarthritis (SpA). Methods Overall, 184 SpA patients were recruited for analyzing diversity of HLA ‐B*27 via an updated high‐resolution polymerase chain reaction amplification with sequence specific primers ( PCR ‐ SSP ). Results The prevalence of HLA ‐B*27 was 94.0%, and four subtypes were identified including HLA ‐B*2704 (77.5%), B*2705 (20.2%), B*2707 (1.7%), and B*2724 (0.6%). There was an obvious male predominance ( P =.05) and markedly elevated C‐reaction protein ( CRP ) in B*27 positive SpA ( P .01). In multivariate linear regression analysis, the elevated CRP was positively associated with HLA ‐B*27 positivity (regression coefficient B=46.1, P =.0003), grade of sacroiliitis (B=47.5, P =.0032), and male gender (B=20.4, P =.0041). Notably, a male predilection was also found in B*2705 positive SpA while B*2707 was associated with older age, higher positive family history, and higher prevalence of extra‐articular features (all P .05). Conclusions In this study, an updated PCR ‐ SSP technique to identify increasing alleles of HLA ‐B*27 was developed and their different effects on clinical manifestations of SpA were demonstrated. Genotyping of HLA ‐B*27 would shed light on our understanding of the pathogenesis of SpA.
机译：背景技术近年来，已经确定了人白细胞抗原B * 27（HLA-B * 27）的不断增加的等位基因。本研究旨在建立HLA -B * 27亚型的更新方法，并研究HLA-B * 27多态性对脊椎炎（SPA）的临床表型的影响。方法总体而言，招募了184例SPA患者，通过使用序列特异性引物（PCR-SSP）的更新的高分辨率聚合酶链反应扩增分析HLA-B * 27的多样性。结果HLA -B * 27的患病率为94.0％，鉴定了四种亚型，包括HLA -B * 2704（77.5％），B * 2705（20.2％），B * 2707（1.7％）和B * 2724（ 0.6％）。存在明显的男性优势（p = .05）并在B * 27正温泉中显着升高的C-反应蛋白（CRP）（P <.01）。在多变量线性回归分析中，升高的CRP与HLA -B * 27阳性正相关（回归系数B = 46.1，P = .0003），骶髂腺度等级（B = 47.5，P = .0032）和男性性别（ b = 20.4，p = .0041）。值得注意的是，在B * 2705阳性水疗中心中也发现了男性偏好，而B * 2707与年龄较大，较高的阳性家族史以及更高的关节特征的患病率（所有P <.05）。结论在该研究中，开发了一种更新的PCR-SSP技术，以鉴定HLA-B * 27增加等位基因，并证明了它们对水疗中心的临床表现的不同影响。 HLA -B * 27的基因分型将阐明我们对SPA发病机制的理解。

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