...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >The deposition and characterization of starch in Brachypodium distachyon
【24h】

The deposition and characterization of starch in Brachypodium distachyon

机译：Broachym Distachyon淀粉的沉积和表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Brachypodium distachyon is a non-domesticated cereal. Nonetheless, Brachypodium was recently introduced as a model plant for temperate cereals. This study compares grain starch metabolism in Brachypodium and barley (Hordeum vulgare). In Brachypodium, we identified and annotated 28 genes involved in starch metabolism and identified important motifs including transit peptides and putative carbohydrate-binding modules (CBMs) of the families CBM20, CBM45, CBM48, and CBM53. Starch content was markedly lower in Brachypodium grains (12%) compared to barley grains (47%). Brachypodium starch granules were doughnut shaped and bimodally distributed into distinct small B-type (2.5-10 mu m) and very small C-type (0.5-2.5 mu m) granules. Large A-type granules, typical of cereals, were absent. Starch-bound phosphate, important for starch degradation, was 2-fold lower in Brachypodium compared with barley indicating different requirements for starch mobilization. The amylopectin branch profiles were similar and the amylose content was only slightly higher compared with barley cv. Golden Promise. The crystallinity of Brachypodium starch granules was low (10%) compared to barley (20%) as determined by wide-angle X-ray scattering (WAXS) and molecular disorder was confirmed by differential scanning calorimetry (DSC). The expression profiles in grain for most genes were distinctly different for Brachypodium compared to barley, typically showing earlier decline during the course of development, which can explain the low starch content and differences in starch molecular structure and granule characteristics. High transitory starch levels were observed in leaves of Brachypodium (2.8% after 14 h of light) compared to barley (1.9% after 14 h of light). The data suggest important pre-domesticated features of cereals.
机译：Broachypodium distachyon是一种非驯化谷物。尽管如此，最近介绍了颅卷曲作为温带谷物的模型植物。该研究比较了粒淀粉藻和大麦（Hordeum Vulgare）的谷物淀粉代谢。在刷卷曲中，我们鉴定和注释了参与淀粉代谢的28个基因，并确定了家族CBM20，CBM45，CBM48和CBM53的过渡肽和推定碳水化合物结合模块（CBMS）的重要基因。与大麦颗粒（47％）相比，淀粉含量明显降低粒状粒子（12％）。将铜卷曲淀粉颗粒甜甜圈形状和双峰分布成不同的小B型（2.5-10μm），非常小的C型（0.5-2.5μm）颗粒。没有典型的谷物的大型颗粒。不存在。与淀粉降解的淀粉结合磷酸盐，与大麦的淀粉酞薄的淀粉降低是2倍，表明淀粉动员的不同要求。与大麦CV相比，支链淀粉分支型材相似，并且直链淀粉含量略高。金色承诺。与大角X射线散射（蜡）测定的大麦（20％）相比，铜卷曲淀粉颗粒的结晶度低（10％），通过差示扫描量热法（DSC）确认分子障碍。与大麦相比，大多数基因大多数基因的谷物中的表达谱明显不同，通常在发育过程中显示出早期的下降，这可以解释淀粉分子结构和颗粒特性的低淀粉含量和差异。与大麦（14小时后14小时后的2.8％）观察到高暂性淀粉水平（14小时后的2.8％）（1.9％在14小时后）。数据表明了谷物的重要预驯化特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号