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首页> 外文期刊>Journal of Experimental Botany >PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana
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PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana

机译：perk-kipk-kcbp信号负面调节拟南芥的根系生长

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摘要

The Arabidopsis proline-rich, extensin-like receptor-like kinases (PERKs) are a small group of receptor-like kinases that are thought to act as sensors at the cell wall through their predicted proline-rich extracellular domains. In this study, we focused on the characterization of a subclade of three Arabidopsis predicted PERK genes, PERK8, -9, and -10, for which no functions were known. Yeast two-hybrid interaction studies were conducted with the PERK8,-9, and -10 cytosolic kinase domains, and two members of the Arabidopsis AGC VIII kinase family were identified as interacting proteins: AGC1-9 and the closely related kinesin-like calmodulin-binding protein (KCBP)-interacting protein kinase (KIPK). As KIPK has been identified previously as an interactor of KCBP, these interactions were also examined further and confirmed in this study. Finally, T-DNA mutants for each gene were screened for altered phenotypes under different conditions, and from these screens, a role for the PERK, KIPK, and KCBP genes in negatively regulating root growth was uncovered.
机译：拟南芥富含富含的延伸的受体样激酶（Perks）是一小组受体样激酶，被认为是通过其预测的脯氨酸细胞外结构域作为细胞壁的传感器。在这项研究中，我们专注于三个拟南芥预测的PERK基因，PERK8，-9和-10的表征，没有任何功能。用Perk8，-9和-10个细胞溶质激酶结构域进行酵母双杂化相互作用研究，拟南芥AGC VIII激酶家族的两个成员被鉴定为相互作用蛋白质：AGC1-9和密切相关的Kinesin样钙调蛋白 - 结合蛋白（KCBP） - 交互式蛋白激酶（KIPK）。由于以前作为KCBP的交互式鉴定的Kipk，还在本研究中进一步检查并确认这些相互作用。最后，将每个基因的T-DNA突变体筛选在不同条件下的改变的表型，并且从这些屏幕中，揭开了百分症调节根系生长的PERK，KIPK和KCBP基因的作用。

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