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首页> 外文期刊>Journal of Experimental Botany >Overexpression of OsPGIP2 confers Sclerotinia sclerotiorum resistance in Brassica napus through increased activation of defense mechanisms
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Overexpression of OsPGIP2 confers Sclerotinia sclerotiorum resistance in Brassica napus through increased activation of defense mechanisms

机译：OSPGIP2的过度表达通过增加防御机制的激活来赋予甘蓝型油菜中的Sclerotinia scleriorum抗性

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摘要

Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is the most serious disease affecting the yield of the agriculturally and economically important crop Brassica napus (rapeseed). In this study, Oryza sativa polygalacturonase-inhibiting protein 2 (OsPGIP2) was found to effectively enhanced rapeseed immunity against S. sclerotiorum infection. Leaf extracts of B. napus plants overexpressing OsPGIP2 showed enhanced S. sclerotiorum resistance by delaying pathogen infection. The constitutive expression of OsPGIP2 in rapeseed plants provided a rapid and effective defense response, which included the production of reactive oxygen species, interactions with S. sclerotiorum polygalacturonases (SsPG3 and SsPG6), and effects on the expression of defense genes. RNA sequencing analysis revealed that the pathogen induced many differentially expressed genes associated with pathogen recognition, redox homeostasis, mitogen-activated protein kinase signaling cascades, hormone signaling pathways, pathogen-/defense-related genes, and cell wall-related genes. The overexpression of OsPGIP2 also led to constitutively increased cell wall cellulose and hemicellulose contents in stems without compromising seed quality. The results demonstrate that OsPGIP2 plays a major role in rapeseed defense mechanisms, and we propose a model for OsPGIP2-conferred resistance to S. sclerotiorum in these plants.
机译：由Sclerotinia Sclerotiorum引起的Sclerotinia茎腐（SSR）是影响农业和经济上重要的作物芸苔（油菜籽）产量的最严重的疾病。在本研究中，发现Oryza sativa polygalactulonase抑制蛋白2（OSPgip2）有效地增强了针对S.Sclerotiorum感染的菜籽梗阻。叶片提取物的叶片植物过表达OSPGIP2显示出通过延迟病原体感染而增强的S.Scleriorum抗性。 OSPGIP2在菜籽油中的组成型表达提供了一种快速有效的防御反应，包括生产活性氧，与S.Spg3和SSPG6）的相互作用，以及对外防基因表达的影响。 RNA测序分析表明，病原体诱导了许多与病原体识别，氧化还原稳态，丝裂剂活化蛋白激酶信号级联，激素信号传导途径，病原体/防御相关基因和细胞壁相关基因相关的差异表达基因。 OSPGIP2的过表达也导致茎中的细胞壁纤维素和半纤维素含量而不会损害种子质量。结果表明，OSPGIP2在油菜防御机制中发挥着重要作用，我们提出了对这些植物中的OSPGIP2赋予S.Sclerotiorum的抵抗型号。

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