...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments
【24h】

Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments

机译：靶向体内叶绿体内包膜膜的多孔膜蛋白涉及多种跨膜段

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The inner envelope membrane (IEM) of the chloroplast plays crucial roles in forming an osmotic barrier and controlling metabolite exchange between the organelle and the cytosol. The IEM therefore harbours a number of membrane proteins and requires the import and integration of these nuclear-encoded proteins for its biogenesis. Recent studies have demonstrated that the transmembrane segment of single-spanning IEM proteins plays key roles in determining their IEM localization. However, few studies have focused on the molecular mechanisms by which polytopic membrane proteins are targeted to the IEM. In this study, we investigated the targeting mechanism of polytopic IEM proteins using the protein Cor413im1 as a model substrate. Cor413im1 does not utilize a soluble intermediate for its targeting to the IEM. Furthermore, we show that the putative fifth transmembrane segment of Cor413im1 is necessary for its targeting to the IEM. The C-terminal portion containing this transmembrane segment is also able to deliver Cor413im1 protein to the IEM. However, the fifth transmembrane segment of Cor413im1 itself is insufficient to target a fusion protein to the IEM. These data suggest that the targeting of polytopic membrane proteins to the chloroplast IEM in vivo involves multiple transmembrane segments and that chloroplasts have evolved a unique mechanism for the integration of polytopic proteins to the IEM.
机译：叶绿体的内包络膜（IEM）在形成渗透屏障和控制细胞器和细胞溶胶之间的代谢物交换时起着至关重要的作用。因此，IEM暂时存在许多膜蛋白，并且需要这些核编码蛋白的进口和整合其生物发生。最近的研究表明，单一跨越IEM蛋白的跨膜段在确定其IEM本地化时起着关键作用。然而，很少有研究专注于多种多纤膜蛋白靶向IEM的分子机制。在这项研究中，我们研究了使用蛋白质COR413IM1作为模型基质的多孔IEM蛋白的靶向机制。 Cor413im1不利用可溶性中间体来靶向IEM。此外，我们表明，COR413IM1的推定第五跨膜段是其靶向IEM的必要条件。含有该跨膜段的C末端部分也能够向IEM递送COR413IM1蛋白。然而，Cor413im1本身的第五跨膜段不足以将融合蛋白靶向IEM。这些数据表明，多粒膜蛋白在体内致氯化体IEM的靶向涉及多种跨膜段，并且叶绿体已经演化了一种独特的机制，用于将多种子蛋白与IEM集成的独特机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号