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首页> 外文期刊>Journal of genetics >Cloning and expression analysis of a new anther-specific gene CaMF4 in Capsicum annuum
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Cloning and expression analysis of a new anther-specific gene CaMF4 in Capsicum annuum

机译：辣椒囊肿中新的植物特异性基因CAMF4的克隆及表达分析

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摘要

Our previous study on the genic male sterile-fertile line 114AB of Capsicum annuum indicated a diversity of differentially expressed cDNA fragments in fertile and sterile lines. In this study, a transcript-derived fragment (TDF), male fertile 4 (CaMF4) was chosen for further investigation to observe that this specific fragment accumulates in the flower buds of the fertile line. The full genomic DNA sequence of CaMF4 was 894 bp in length, containing two exons and one intron, and the complete coding sequence encoded a putative 11.53 kDa protein of 109 amino acids. The derived protein of CaMF4 shared similarity with the members of PGPS/D3 protein family. The expression of CaMF4 was detected in both the flower buds at stage 8 and open flowers of the male fertile line. In contrast to this observation, expression of CaMF4 was not detected in any organs of the male sterile line. Further analysis revealed that CaMF4 was expressed particularly in anthers of the fertile line. Our results suggest that CaMF4 is an anther-specific gene and might be indispensable for anther or pollen development in C. annuum.
机译：我们对辣椒的基因雄性无菌 - 肥沃线114ab的研究表明了差异表达的育种和无菌线的多样性。在该研究中，选择转录物衍生的片段（TDF），雄性育4（CAMF4）进行进一步研究，以观察到这种特定的片段积聚在肥沃线的花蕾中。 CAMF4的全基因组DNA序列长度为894bp，含有两个外显子和一个内含子，并且完全编码序列编码了109个氨基酸的推定的11.53kDa蛋白。 CAMF4的衍生蛋白与PGPS / D3蛋白家族的成员共享相似性。在第8阶段的花蕾中检测CAMF4的表达，并打开雄性肥沃线的花朵。与该观察结果相比，在雄性无菌线的任何器官中未检测到CAMF4的表达。进一步的分析表明，CAMF4特别是在肥沃线的花茎中表达。我们的研究结果表明，CAMF4是特定于特定的基因，对于C. Anrum的花粉或花粉发育可能是必不可少的。

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