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首页> 外文期刊>Journal of genetics >The Drosophila bipectinata species complex: phylogenetic relationship among different members based on chromosomal variations
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The Drosophila bipectinata species complex: phylogenetic relationship among different members based on chromosomal variations

机译：果蝇Bipectinata种族复合物：基于染色体变化的不同成员之间的系统发育关系

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Making interspecific hybridizations, where possible remains an unparalleled option for studying the intricacies of speciation. In the Drosophila bipectinata species complex comprising of four species, namely D. bipectinata, D. parabipectinata, D. malerkotliana and D. pseudoananassae, interspecific hybrids can be obtained in the laboratory, thus bequeathing an ideal opportunity for studying speciation and phylogeny. With the view of investigating the degree of divergence between each species pair, we planned to study the polytene chromosomes of the F (1) hybrids, as it would mirror the level of compatibility between the genomes of the parental species. Two sets of crosses were made, one involving homozygous strains of all four species from India and the other including homozygous strains from different places across the globe. Polytene chromosomes of F (1) larvae from both sets of crosses had similar configurations. In F (1) larvae from crosses involving D. bipectinata, D. parabipectinata and D. malerkotliana, complex configurations (depicting overlapping inversions) could be detected in different arms. However, they were fairly synapsed, indicating that the differences are only at the level of gene arrangements. The polytene chromosomes of larvae obtained by crossing D. pseudoananassae with the other three species were very thin with gross asynapsis in all the arms, demonstrating that the genome of D. pseudoananassae is widely diverged from rest of the species. The overlapping inversions (reflected in complex configuration), are inferred in the light of earlier chromosomal studies performed in this complex.
机译：制定三种杂交，在可能仍然是研究物种复杂性的无与伦比的选择。在包含四种物种的果蝇Bipectinata络合物中，即D. Bipectinata，D. parabipectinata，D.Malerkotliana和D.Pseudoananassae，可以在实验室中获得三种杂种，因此遗留了学习物种和系统发育的理想机会。凭借调查每种物种对之间的分歧程度，我们计划研究F（1）杂种的重聚乙烯染色体，因为它将反映家长物种的基因组之间的相容性水平。制作了两套十字架，其中涉及来自印度的所有四种物种的纯合子菌株，另一个包括来自全球各地的不同地方的纯合菌株。来自两组横梁的F（1）幼虫的聚对染色体具有相似的构造。在F（1）中，来自涉及D. Bipectinata，D. parabipectinata和D.Malerkotliana的幼虫，可以在不同的臂中检测到复杂的配置（描绘重叠的逆分）。但是，它们相当突触，表明差异仅在基因安排的水平处。通过穿越D.幼虫的幼虫与其他三种物种的聚苯染色体，在所有臂中粗糙的脂肪，表明D.Pseudoananassae的基因组广泛偏离了物种的其余部分。根据在该复合物中进行的早期染色体研究，推断重叠逆转（反映在复杂的配置中）。

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