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首页> 外文期刊>Journal of genetics >Identification of virulence factors and type III effectors of phylotype I, Indian Ralstonia solanacearum strains Rs-09-161 and Rs-10-244
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Identification of virulence factors and type III effectors of phylotype I, Indian Ralstonia solanacearum strains Rs-09-161 and Rs-10-244

机译：杀虫因子和型毒性因子的鉴定，III型I，印度红斑狼疮菌株RS-09-161和RS-10-244

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摘要

Ralstonia solanacearum is a well-known phytopathogen causing bacterial wilt in a large number of agriculturally important crops. The pathogenicity of R. solanacearum is expressed due to the presence of various virulence factors and effector proteins. In this study, various virulence factors and type III effector proteins of R. solanacearum that are present in the strains Rs-09-161 and Rs-10-244 were identified through bioinformatics approach and compared with other reference strains. R. solanacearum strains, Rs-09-161 and Rs-10-244 belong to the phylotype I, biovar3, and are the only sequenced strains from India infecting solanaceous vegetables. Similarity matrix obtained by comparing the sequences of virulence genes of Rs-09-161 and Rs-10-244 with other reference strains indicated that Rs-09-161 and Rs-10-244 share more than 99% similarity between them and are closely related to GMI1000. The virulence factors in R. solanacearum appear to be highly conserved in the R. solanacearum species complex. Rs-09-161 has 72 type III effectors whereas Rs-10-244 has 77. Comparison of the complete genes of type III effectors of Rs-09-161, Rs-10-244 and GMI1000 revealed the presence of 60 common effectors within them. Further, Rs-09-161 has two unique effectors and Rs-10-244 has four unique effectors. Phylogenetic trees of RipA, RipG, RipH and RipS effector sequences resulted in the grouping of the isolates based on their phylotypes. Group 1 consists of strains that belong to phylotype I including Rs-09-161 and Rs-10-244. Phylotype III strain CMR15 forms a group closely associated with phylotype I. The strains belonging to phylotypes II and IV have separated to form two different groups.
机译：Ralstonia solanacearum是一种众所周知的植物病理原因，导致大量农业上的细菌枯萎病。由于各种毒力因子和效应蛋白存在，表达了R.Solanacearum的致病性。在该研究中，通过生物信息学方法鉴定菌株RS-09-161和RS-10-244中存在的各种毒力因子和R.Solanacearum的III型效应蛋白并与其他参考菌株进行比较。 R.Solanacearum菌株，RS-09-161和RS-10-244属于Phylotype I，Biovar3，并且是来自印度感染溶律蔬菜的唯一测序菌株。通过将RS-09-161和RS-10-244的毒力基因序列与其他参考菌株进行比较来获得的相似性矩阵表明RS-09-161和RS-10-244之间的相似性超过99％并密切相关与GMI1000相关。 R.Solanacearum的毒力因子似乎在R.Solanacearum物种复合物中高度保守。 RS-09-161具有72型III型效应器，而RS-10-244具有77. rs-09-161，RS-10-244和GMI1000的III型效应器的完整基因的比较显示出60个常见效应的存在他们。此外，RS-09-161具有两个独特的效果，RS-10-244具有四个独特的效果。 RIPA，RIPG，RIPH和裂隙效应序列的系统发育树导致基于它们的种植型分组分离物。第1组包括属于PhyLotype I的菌株，包括RS-09-161和RS-10-244。 Phylotype III菌株CMR15形成与PhyLotype I密切相关的组。属于Phylotypes II和IV的菌株已分开以形成两个不同的基团。

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