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首页> 外文期刊>Journal of genetics >Marker-aided selection and validation of various Pi gene combinations for rice blast resistance in elite rice variety ADT 43
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Marker-aided selection and validation of various Pi gene combinations for rice blast resistance in elite rice variety ADT 43

机译:精英稻品种ADT 43的稻瘟病耐水稻耐药组合的标记选择及验证

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摘要

Rice blast caused by fungal pathogen Pyricularia oryzae has a major impact on reducing yield potential of rice. In this study, homozygous plants were selected using microsatellite markers from the BC3F2 population pyramided with four major genes in elite rice variety ADT 43. Background and selected lines with various blast resistance gene combinations were screened under natural conditions to study the effects of various gene combinations. Upon inspection of lines with different gene combinations, the three-gene pyramided line Pi54+Pi33+Pi1 was found to be highly resistant with the score of 3.3 followed by other three-gene pyramided lines Pi54+Pi2+Pi1 and Pi33+Pi2+Pi1, with the scores of 3.9 and 3.8, respectively. Two-gene pyramided lines Pi54+Pi1, Pi33+Pi1 and Pi2+Pi1 were found to be moderately resistant with a mean score of 4.0 each. In the case of monogenic lines, positive plants for Pi54 performed almost equal to three-gene pyramided lines with a mean score of 3.6. Lines with Pi2 and Pi1 were found to be moderately resistant and moderately susceptible with the mean scores of 4.1 and 4.5, respectively.
机译:由真菌病原体肌肉菌造成的稻瘟病患者对降低水稻产量潜力的重大影响。在该研究中,使用来自BC3F2种群的微卫星标记选择纯合的植物,从BC3F2种群中含有四个主要基因在Elite Rice品种ADT 43中。在自然条件下筛选出具有各种抗抗性基因组合的背景和选定的线,以研究各种基因组合的影响。在用不同基因组合检查线时,发现三种基因的显着线PI54 + PI33 + PI1具有高度抗性,其得分为3.3,其次是其他三基因大茂碱基PI54 + PI2 + PI1和PI33 + PI2 + PI1 ,分别为3.9和3.8。发现两种基因大叠线PI54 + PI1,PI33 + PI1和PI2 + PI1是适度抗性,平均得分为4.0。在单一的线性线的情况下,PI54的阳性植物几乎等于三基本的显着线,平均得分为3.6。发现具有PI2和PI1的系列以分别为4.1和4.5的平均评分来适度抵抗和中度易感。

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