Improvement of hydroxyapatite-coated nonwoven fabrics by coating with silk fibroin for use

Yohko Gotoh; Shingo Niimi; Tomokazu Matsuura; Yasuyuki Ishizuka

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Improvement of hydroxyapatite-coated nonwoven fabrics by coating with silk fibroin for use

机译：用丝素蛋白涂层改善羟基磷灰石涂覆的非织造织物

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We modified hydroxyapatite (HA)-coated nonwoven polyethylene/polypropylene fabrics by coating with silk fibroin (SF) to improve them as a three-dimensional substrate for culturing human hepatocellular carcinoma-derived FLC-5 cells. After 25 days of culture, FLC-5 cells cultured on nonwoven fabrics coated with HA and HA plus SF (HA-SF) partially formed multicellular aggregates, whereas the cells cultured on tissue culture plates formed monolayers. FLC-5 cells cultured on nonwoven fabrics and tissue culture plates for 25 days were subjected to quantitative assay for cell number and albumin secretion. The lowest cell number and the largest amount of albumin were found for nonwoven fabrics coated with HA-SF. Normalizing albumin values to cell number data demonstrated that albumin secretory function per cell on 2 kinds of nonwoven fabrics was remarkably higher than that on tissue culture plates. Moreover, albumin secretory function per cell on nonwoven fabrics coated with HA-SF was twice as high as thaton HA-coated nonwoven fabrics. These preliminary results suggest that modification of HA-coated nonwoven fabrics by SF coating induced functional improvement of FLC-5 cells.

机译：通过用丝素蛋白（SF）涂覆，改性羟基磷灰石（HA）涂覆的非织造聚乙烯/聚丙烯织物，以改善它们作为培养人肝细胞衍生的FLC-5细胞的三维底物。在培养25天后，在涂有HA和HA加SF（HA-SF）的非织造织物上培养FLC-5细胞部分形成的多细胞聚集体，而在组织培养板上培养的细胞形成单层。对非织造织物和组织培养板培养的FLC-5细胞25天进行细胞数和白蛋白分泌的定量测定。发现最低的细胞数和最大量的白蛋白用于涂有HA-SF的非织造织物。将白蛋白值标准化为细胞数数据表明，每电池的白蛋白分泌功能在2种非织造织物上显着高于组织培养板上的蛋白质分泌功能。此外，涂有HA-SF的非织造织物上的白蛋白分泌功能是HA-涂覆的非织造织物的两倍。这些初步结果表明，通过SF涂层诱导FLC-5细胞的功能改进，通过SF涂层改变HA涂覆的非织造织物。

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来源
《Journal of insect biotechnology and sericology》 |2017年第1期|共5页
作者
Yohko Gotoh; Shingo Niimi; Tomokazu Matsuura; Yasuyuki Ishizuka;
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作者单位

Institute of Agrobiological Sciences National Agriculture and Food Research Organization 1-2 Ohwashi Tsukuba Ibaraki 305-8634 Japan;

Division of Biological Chemistry and Biologicals National Institute of Health Sciences 1-18-1 Kamiyoga Setagaya-ku Tokyo 158-8501 Japan;

Department of Laboratory Medicine The Jikei University School of Medicine 3-25-8 Nishi-shinbashi Minato-ku Tokyo 105-8461 Japan;

BioFuture Technologies Ltd. 3-17-15 Shiba Minato-ku Tokyo 105-0014 Japan;

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原文格式 PDF
正文语种 eng
中图分类蚕桑;
关键词
hepatic tissue engineering; nonwoven fabrics; coating; silk fibroin;

机译：肝组织工程;无纺布;涂层;丝素蛋白;

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专利

1. Improvement of hydroxyapatite-coated nonwoven fabrics by coating with silk fibroin for use as a scaffold for culture of human hepatocellular carcinoma-derived FLC-5 cells [J] . Yohko Gotoh, Shingo Niimi, Tomokazu Matsuura, Journal of insect biotechnology and sericology . 2017,第1期

机译：通过用丝素蛋白包被用作人肝细胞癌衍生的FLC-5细胞培养的支架，改进羟基磷灰石涂层的无纺布
2. Comparisons between silk fibroin nonwoven electrospun fabrics using aqueous and formic acid solutions [J] . Kishimoto Yuki, Kobashi Takanori, Yamanaka Shigeru, International Journal of Polymeric Materials . 2018,第6a9期

机译：使用含水和甲酸溶液丝纤维素非织造织物织物的比较
3. A New Consolidation System for Aged Silk Fabrics: Interaction between Ethylene Glycol Diglycidyl Ether, Silk Fibroin and Artificial Aged Silk Fabrics [J] . Ziying Wu, Dun Huang, Zhiwen Hu Fibers and Polymers . 2014,第6期

机译：老化的真丝织物的新固结系统：乙二醇二缩水甘油醚，丝素蛋白与人工老化的真丝织物之间的相互作用
4. Silk Fabric Protection Obtained via Chemical Conjugation Transglutaminase and Silk Fibroin Reinforcement [C] . Suhua Zhao, Hongling Liu, Weidong Yu Sino-Africa international symposium on textiles and apparel . 2018

机译：通过化学缀合转谷氨酰胺酶和丝素蛋白增强获得丝绸织物保护
5. The characterization of the initial degradative alterations of Bombyx mori silk fibroin: A comparative study of artificially aged silk fabric and silk fabrics from The First Ladies Collection, Smithsonian Institution, Washington, D.C. [D] . Becker, Mary Ann. 1993

机译：家蚕丝素蛋白初始降解变化的表征：华盛顿特区史密森学会的The First Ladies Collection中人工老化的真丝织物和真丝织物的比较研究。
6. Surface Modification and Characterisation of Silk Fibroin Fabric Produced by the Layer-by-Layer Self-Assembly of Multilayer Alginate/Regenerated Silk Fibroin [O] . Gaotian Shen, Xingyou Hu, Guoping Guan, -1

机译：多层藻酸盐/再生丝素蛋白逐层自组装产生的丝素蛋白织物的表面改性和表征
7. Surface Modification and Characterisation of Silk Fibroin Fabric Produced by the Layer-by-Layer Self-Assembly of Multilayer Alginate/Regenerated Silk Fibroin. [O] . Gaotian Shen, Xingyou Hu, Guoping Guan, 2015

机译：多层海藻酸钠/再生丝素蛋白层层自组装生产丝素蛋白织物的表面改性及表征。

Improvement of hydroxyapatite-coated nonwoven fabrics by coating with silk fibroin for use

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