...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology and Applied Biochemistry >Genome shuffling in the ethanologenic yeast Candida krusei to improve acetic acid tolerance
【24h】

Genome shuffling in the ethanologenic yeast Candida krusei to improve acetic acid tolerance

机译：产乙醇酵母假丝酵母念珠菌中的基因组改组以提高乙酸耐受性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genome shuffling was used to improve the acetic acid tolerance of an ethanologenic yeast, Candida krusei GL560. A mutant, S4-3, was isolated and selected after four rounds of genome shuffling. It was found that the mutant S4-3 had a higher viability in the YNBX (yeast nitrogen base/xylose) medium with acetic acid and grew better in the YPD (yeast extract, peptone and dextrose) medium [1 % (w/v) yeast extract, 2% (w/v) peptone and 2 % (w/v) glucose] with acetic acid than the parent strain GLS60. The mutant S4-3 also improved its multiple stress tolerance to ethanol, H2O2, heat and freeze-thaw. Furthermore, S4-3 showed higher ethanol production than GL560 in EFM (ethanol fermentation medium) with or without acetic acid. The DNA content of S4-3 was similar to its parent strains in the genome shuffling. This suggested that gene exchange, as caused by homologous recombination, may have occurred during the process. Higher membrane integrity and intracellular catalase activity were two possible reasons for the higher acid-tolerance phenotype of S4-3. These results indicated that genome shuffling is a powerful means of rapidly improving the complex traits of non-haploid organisms, while still maintaining robust growth.
机译：基因组改组用于提高产乙醇酵母克鲁斯假丝酵母GL560的耐乙酸性。经过四轮基因组改组后，分离并选择了一个突变体S4-3。发现突变体S4-3在具有乙酸的YNBX（酵母氮碱/木糖）培养基中具有更高的生存力，并且在YPD（酵母提取物，蛋白和右旋糖）培养基中生长更好[1％（w / v）酵母提取物，2％（w / v）蛋白ept和2％（w / v）葡萄糖]比亲本菌株GLS60加乙酸。突变体S4-3还提高了其对乙醇，H2O2，热和冻融的多重胁迫耐受性。此外，在有或没有乙酸的情况下，S4-3在EFM（乙醇发酵培养基）中显示出比GL560高的乙醇产量。在基因组改组中，S4-3的DNA含量与其亲本菌株相似。这表明，在同源重组过程中可能发生了由同源重组引起的基因交换。较高的膜完整性和细胞内过氧化氢酶活性是S4-3耐酸表型较高的两个可能原因。这些结果表明，基因组改组是快速改善非单倍体生物体复杂特征的强大手段，同时仍保持了强劲的增长。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号