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首页> 外文期刊>Journal of Helminthology >Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected rats
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Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected rats

机译：对均多雌性女性的特异性IgG和免疫复合反应委内瑞斯的丙烯酮酮委内瑞斯委内瑞斯诊断受感染大鼠免疫抑制作用

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摘要

In the present study, antigens from parthenogenetic females and eggs of Strongyloides venezuelensis, or anti-parthenogenetic-female and anti-egg antigens were used to detect specific IgG and immune complex responses, respectively. Serum samples from experimentally infected immunocompetent and immunosuppressed rats were analysed on days 5, 8, 13 and 21 post-infection (dpi). An enzyme-linked immunosorbent assay (ELISA) was performed using alkaline parasite extract for specific IgG detection, and anti-parthenogenetic-female or anti-egg antigens for immune complex detection. The data were analysed using analysis of variance (ANOVA), followed by a Bonferroni test. When parthenogenetic female or egg extracts were used as antigens, specific IgGs were not detected in either immunocompetent or immunosuppressed rats. When anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used, immune complexes were detected for the duration of the infection in immunosuppressed animals and were only detected between 5 and 13 dpi in immunocompetent animals. The duration of infection was not significantly different between the immunocompetent and immunosuppressed groups when anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used. Parthenogenetic female extracts yielded significant differences between antibody and immune complex responses in immunocompetent rats from 5 to 13 dpi, but only on day 5 dpi in immunosuppressed rats. Exposure to S. venezuelensis egg extract yielded significant differences in both antibody and immune complex detection between immunocompetent and immunosuppressed rats for the duration of the infection. In conclusion, ELISA using alternative antigens may be a successful strategy for identifying immune complexes in serum samples and diagnosing active strongyloidiasis, particularly under conditions of immunosuppression.
机译：在本研究中，用于分别检测特异性IgG和免疫复合反应的副雌性女性和抗孢子醛雌性和抗蛋抗原的抗原。在感染后第5,8,13和21天分析来自实验感染的免疫活性和免疫抑制大鼠的血清样品。使用碱性寄生虫提取物进行酶联免疫吸附测定（ELISA），用于特异性IgG检测，以及用于免疫复合物检测的抗单性雌性或抗蛋抗原。使用差异分析（ANOVA）分析数据，然后进行Bonferroni测试。当使用单性雌性或蛋提取物作为抗原时，在免疫活性剂或免疫抑制的大鼠中未检测到特异性IgG。当抗单性发生 - 女性或抗-s。使用委内瑞式茄子，在免疫抑制动物中感染的持续时间检测免疫复合物，并且仅在免疫活性动物中检测到5-13 dpi。在抗替代 - 女性或抗-S的免疫活性剂和免疫抑制基团之间的感染持续时间没有显着差异。使用委内瑞式蛋。单向性女性提取物在5至13dPi的免疫活性大鼠中产生抗体和免疫复合反应之间的显着差异，但仅在免疫抑制大鼠的第5天DPI上。接触S.委内瑞式蛋提取物在免疫活性剂和免疫抑制大鼠之间的抗体和免疫复合物检测中产生显着差异，在感染的持续时间内。总之，使用替代抗原的ELISA可以是用于在血清样品中鉴定免疫复合物并诊断活性抗硫综合体，特别是在免疫抑制条件下的成功策略。

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