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首页> 外文期刊>Journal of Veterinary Research >Potential role of macrophage migration inhibitory factor in the pathogenesis of Marek's disease
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Potential role of macrophage migration inhibitory factor in the pathogenesis of Marek's disease

机译：巨噬细胞迁移抑制因子在Marek疾病发病机制中的潜在作用

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Introduction: Marek's disease virus (MDV) can cause malignant T-cell lymphomas and immunosuppression in chickens. Macrophage migration inhibitory factor (MIF) not only plays a critical role in inhibiting T-cell responses, but also contributes to multiple aspects of tumour progression. The aim of this study was to reveal the potential role of MIF in the pathogenesis of MDV infection. Material and Methods: MIF gene expression levels were measured by using real-time PCR. Expression was assayed at different times in chicken embryo fibroblast (CEF) cells and tissue samples of SPF chickens infected with different MDV strains and fold change was calculated by the 2-Delta Delta(CT) method. Results: The expression of MIF was significantly downregulated (p < 0.05 and FC > 2) in CEF cells infected with the very virulent MDV RB1B strain at 48 h post infection (hpi) and in the skin and spleen at 14 days post infection (dpi). The reduction of MIF expression was also found in CEF cells infected by reticuloendotheliosis virus (REV), avian leukosis virus subgroup J (ALV-J), and MDV vaccine strain CVI988 or in HD11 cells stimulated with TLR2, 3, 4, and 7 ligands. Interestingly, MIF expression decreased continuously from 7 to 28 dpi in the thymus after RB1B virus infection while it increased after CVI988 virus infection. Upregulated expression of MIF was found in CEF infected with RB1B at 96 hpi and in the spleen and skin at 21 and 28 dpi. Conclusion: The present study revealed the different expression pattern of MIF in response to MDV infection and indicated that MIF level may be associated with MDV pathogenesis.
机译：简介：MAREK的疾病病毒（MDV）可导致恶性T细胞淋巴瘤和鸡免疫抑制。巨噬细胞迁移抑制因子（MIF）不仅在抑制T细胞反应方面发挥着关键作用，而且还有助于肿瘤进展的多个方面。本研究的目的是揭示MIF在MDV感染发病机制中的潜在作用。材料和方法：通过使用实时PCR测量MIF基因表达水平。在鸡胚成纤维细胞（CEF）细胞（CEF）细胞（CEF）细胞中测定表达，并通过2Δδ（CT）法计算感染不同MDV菌株的SPF鸡的组织样品和折叠变化。结果：MIF的表达在感染48小时内（HPI）和皮肤和脾脏中感染的CEF细胞中的显着下调（P <0.05和FC> 2），在感染后14天，皮肤和脾脏（DPI ）。 MIF表达的还原在由网状细胞病毒（Rev），禽类白血病病毒亚组J（ALV-J）和MDV疫苗菌株CVI988或用TLR2,3,4和7个配体刺激的HD11细胞中的CEF细胞中发现。有趣的是，在CVI988病毒感染后，MIF表达在RB1B病毒感染后，在胸腺量中连续减少7至28dPI。在96 HPI的CEF中发现MIF的上调表达在96 HPI和21和28dPI的脾脏和皮肤中发现。结论：本研究揭示了MIF响应于MDV感染的不同表达模式，并表明MIF水平可以与MDV发病机制有关。

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