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Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model

机译：豚鼠模型中的消化路径C. Burnetii传输的可能性评价

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Introduction: Q fever (coxiellosis) is an infectious disease of animals and humans, caused by C. burnetii and widely distributed throughout the world. It is known that people and animals acquire the disease predominantly via inhalation of infectious aerosols. The possibility of transmission of the pathogen by the alimentary route is still a matter of debate and remains controversial. Therefore the aim of this study was to fill the gaps in knowledge of oral transmission of C. burnetii by conducting biological tests on the guinea pig model. Material and Methods: Guinea pigs, divided into five groups comprising a negative control and four experimental groups, received specified concentrations of C. burnetii per os. To determine the presence of specific antibodies, blood samples were tested using CFT. Also, internal organs collected during necropsy were screened by a real-time PCR targeting IS1111. Additionally, histopathological evaluation of the tissues was performed. Results: The presence of antibodies and pathogen DNA in caecum was confirmed in one guinea pig from experimental group IV. C. burnetii was also detected in testicular tissue collected from one animal of experimental group II. Conclusions: The presence of pathogen DNA in the testicular tissue indicates that infection spreads haematogenously. In the majority of experimental animals specific antibodies and genetic material of C. burnetii were not detected. This fact suggests that development of infection depends on many factors, such as animal immune status.
机译：介绍：Q发烧（Coxiellosis）是动物和人类的传染病，由C.Burnetii引起并广泛分布在全世界。众所周知，人们和动物主要通过吸入传染性气溶胶来获得疾病。通过消化道传播病原体的可能性仍然是辩论的问题，并且仍然存在争议。因此，本研究的目的是通过在豚鼠模型上进行生物学测试，填补燃烧伯类伯爵的口服透射差距。材料和方法：豚鼠，分为包含阴性对照和四组的五组，每种OS接受指定浓度的C.Burnetii。为了确定特异性抗体的存在，使用CFT测试血液样品。此外，通过真正的PCR靶向筛选尸检期间收集的内脏器官IS1111。另外，进行组织的组织病理学评估。结果：在实验组IV的一只豚鼠中证实了盲肠中抗体和病原体DNA的存在。在从一只实验组II的一只动物收集的睾丸组织中也检测到伯纳汀。结论：睾丸组织中病原体DNA的存在表明感染促进血液发生。在大多数实验动物中，未检测到C.Curnetii的特异性抗体和遗传物质。这一事实表明，感染的发展取决于许多因素，例如动物免疫状态。

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