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首页> 外文期刊>Journal of Veterinary Research >Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves
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Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves

机译：基于Saponin的支原体Bovis疫苗含有溶菌酶二聚体佐剂刺激犊牛的急性相响应

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Introduction: Mycoplasma bovis is known as a causative agent of many disorders in cattle. In Europe, there is still a lack of commercial vaccines against M. bovis infection. Acute phase response (APR) is a non-specific host reaction to infection, most seen in changes in production of acute phase proteins. The aim of this study was to analyse APR in calves administered with an experimental M. bovis vaccine. Material and Methods: Twelve healthy female calves were divided into two equal groups: experimental and control. The experimental vaccine containing the field M. bovis strain and two adjuvants such as saponin and lysozyme dimer was subcutaneously administered to the experimental group. Phosphate buffered saline was taken as the placebo and given to the control group by the same route as the vaccine. Blood samples were collected prior to the study (day 0), then daily up to day 7, and then each seven days until day 84 post vaccination. The concentrations of serum amyloid A (SAA), haptoglobin (Hp), interferon-gamma (IFN-gamma), and inteleukin-4 (IL-4) were determined using commercial ELISA kits. Results: Following the vaccination, a significant increase in SAA, Hp, and IFN-gamma concentrations was observed when compared to the unvaccinated calves, whereas the IL-4 concentration was not detectable. Conclusion: The experimental saponin-based M. bovis vaccine containing lysozyme dimer adjuvant visibly stimulated the APR in the calves, and some specific cytokines (Th1 -dependent) directly involved in this response.
机译：介绍：支原体Bovis被称为牛中许多疾病的致病因子。在欧洲，仍然缺乏针对BOVIS感染的商业疫苗。急性阶段反应（APR）是对感染的非特异性宿主反应，大多数人在生产急性期蛋白的产生变化中。本研究的目的是分析用实验性M.Bovis疫苗施用的犊牛中的APR。材料和方法：将十二个健康的女性小牛分为两个相等的群体：实验和控制。皮下给予实验组，含有野粒菌株和两个佐剂如皂苷和溶菌酶二聚体的实验疫苗。将磷酸盐缓冲盐水作为安慰剂，并通过与疫苗相同的途径给予对照组。在研究之前收集血液样品（第0天），然后每日早于第7天，然后每七天疫苗接种后84天。使用商业ELISA试剂盒测定血清淀粉样蛋白A（SAA），哈达氟胺（HP），干扰素-1γ（IFN-Gamma）和Inteleukin-4（IL-4）的浓度。结果：疫苗接种后，与未移植的小牛相比，观察到SAA，HP和IFN-Gamma浓度的显着增加，而IL-4浓度则无法检测到。结论：含有溶菌酶二聚体佐剂的实验性皂苷疫苗明显刺激犊牛中的APR，以及直接参与这种反应的一些特异性细胞因子（Th1依赖性）。

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