• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Veterinary Research >Clostridium perfringens spores in Polish honey samples
【24h】

Clostridium perfringens spores in Polish honey samples

机译：Clostridium profringens孢子在波兰蜂蜜样品中

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction: The aim of this study was examination of honey samples collected from apiaries situated in all Polish provinces for occurrence of Clostridium spp., especially C. perfringens. Material and Methods: The study was carried out on 240 honey samples (15 samples/province). Estimation of Clostridium titre, its cultures and C. perfringens isolate characterisation were performed according to the standard PN-R-64791:1994. A multiplex PCR method for detection of genes coding cpa (alpha toxin), cpb (beta), cpb2 (beta 2), etx (epsilon), iap (iota), and cpe (enterotoxin) toxins was used. Results: Clostridium spp. was noticed in 56% (136/240) of samples, and its titres ranged between 0.1 g and 0.001 g. Clostridium perfringens occurrence was evidenced in 27.5% (66/240) of samples. All isolates were classified to toxinotype A. Conclusions: Evidence of a high number of positive samples with occurrence of Clostridium spp. indicates a potential risk to consumers' health. The infective number of Clostridium spp. is unknown; however, the obtained results have shown that a risk assessment on the entire honey harvesting process should be made in order to ensure microbiological safety. Moreover, a detailed study should be undertaken on the antibiotic resistance of C. perfringens isolates from honey samples.
机译：介绍：本研究的目的是从位于所有抛光众省的养蜂物中收集的蜂蜜样品进行检查，用于发生梭菌SPP。，尤其是C.流量。材料和方法：该研究在240蜂蜜样品（15个样品/省）上进行。根据标准PN-R-64791：1994进行酸葡萄滴虫，其培养物和C.的培养物分离表征。使用用于检测CPA（α毒素），CPB（β），CPB2（β2），ETX（EPSILON），IAP（IOTA）和CPE（肠毒素）毒素的基因的多重PCR方法。结果：Clostridium SPP。在56％（136/240）的样品中被注意到，其滴度范围为0.1g和0.001g。产梭菌的产物发生在27.5％（66/240）的样品中证明。所有分离株分类为毒素A.结论：具有梭菌SPP的发生量大量阳性样品的证据。表示消费者健康的潜在风险。 Clostridium SPP的感染数量。未知;然而，所获得的结果表明，应制备对整个蜂蜜收集过程的风险评估，以确保微生物安全性。此外，应对来自蜂蜜样品的C的抗生素抗性进行详细研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号