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首页> 外文期刊>Journal of Veterinary Research >Detection of antibiotic resistance and classical enterotoxin genes in coagulase -negative staphylococci isolated from poultry in Poland
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Detection of antibiotic resistance and classical enterotoxin genes in coagulase -negative staphylococci isolated from poultry in Poland

机译：波兰家禽分离凝固酶 - 凝固酶葡萄球菌的抗生素抗性和典型肠毒素基因

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Introduction: The study sought to characterise antimicrobial resistance among coagulase-negative Staphylococcus ( CNS) species recovered from broiler chickens and turkeys in Poland including the presence of 12 antimicrobial resistance genes and five classical genes of staphylococcal enterotoxins. Material and Methods: A panel of 11 antimicrobial disks evaluated the phenotypic sensitivity of the tested strains to antibiotics. Five multiplex PCR assays were performed using primer pairs for specific detection of antibiotic resistance genes and staphylococcal enterotoxin A to E genes. Results: Selected antimicrobial agent susceptibility testing revealed 100% of such in in vitro conditions to cefoxitin among strains of Staphylococcus sciuri and S. chromogenes. The blaZ (for beta-lactam) and mecA (for methicillin resistance) genes were in 58.3% and 27.5% of strains, respectively. Among genes resistant to tetracyclines, tetK was most frequent. Fewer (CNS) strains showed genes resistant to macrolides, lincosamides, and florfenicol/chloramphenicol. Multiplex PCR for classical enterotoxins (A-E) detected the see gene in two S. hominis strains, while the seb gene producing enterotoxin B was found in one strain of S. epidermidis. Conclusion: CNS strains of Staphylococcus isolated from poultry were either phenotypically or genotypically multidrug resistant. Testing for the presence of the five classical enterotoxin genes showed that CNS strains, as in the case of S. aureus strains, can be a source of food intoxications.
机译：介绍：该研究寻求在波兰肉鸡和火鸡中回收的凝固酶阴性葡萄球菌（CNS）物种中的抗菌药物抗性，包括存在12个抗菌性抗性基因和五种型葡萄球菌肠毒素的古典基因。材料和方法：11个抗菌磁盘面板评估了测试菌株对抗生素的表型敏感性。使用引物对进行五种多重PCR测定，用于特异性检测抗生素抗性基因和葡萄球菌肠毒素A至E基因。结果：选定的抗菌剂易感性试验显示，在葡萄球菌和S.色度的菌株中，在体外条件下揭示了该体外的体外条件。 Blaz（对于β-内酰胺）和MECA（用于甲氧西林抗性）基因分别为58.3％和27.5％的菌株。在对四环素的基因中，Tetk最常见。较少（CNS）菌株显示出对大环内酯，林磷酰胺和氟苯啉/氯霉素抗性的基因。用于典型肠毒素（A-E）的多重PCR检测到两种S. hominis菌株中的参照基因，而产生肠毒素B的SEB基因被发现在一个菌株的S.表皮中。结论：从家禽中分离的葡萄球菌的CNS菌株是表型或基因型多药抗性。测试五种古典肠毒素基因的存在表明CNS菌株，如在金黄色葡萄球菌株的情况下，可以是食物中毒的源。

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