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首页> 外文期刊>感染症学雑誌 >Relationship between time taken from collection of blood to incubation and measurement in whole-blood interferon gamma assay for diagnosis of Mycobacterium tuberculosis infection
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Relationship between time taken from collection of blood to incubation and measurement in whole-blood interferon gamma assay for diagnosis of Mycobacterium tuberculosis infection

机译：从血液收集到孵育和测量的时间之间的关系，用于诊断结核病感染的全血干扰素γ测定

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BACKGROUND: The introduction of second-generation QuantiFERON-TB (QFT) enables the diagnosis of Mycobacterium tuberculosis (TB) infection with high specificity and sensitivity. This in vitro diagnostic test uses 2 TB-specific proteins (ESAT-6 and CFP-10) to stimulate cells in heparinized whole blood and detects interferon-gamma (IFN-gamma) produced from blood cells. When QFT is done in laboratories outside of the hospital, several hours may be required to transport blood samples. We studied the relationship between QFT results and the time taken from collection of blood to incubation (preincubation time). METHODS: Heparinized whole blood drawn from TB suspects was immediately transported to a laboratory. We started to incubate 4 aliquots of blood with ESAT-6, CFP-10, mitogen (phytohemagglutinin), and nil control, at 1, 3, 6, 9, and 12 h after blood was drawn. After incubation, the concentration of IFN-gamma in each plasma sample was determined by ELISA, and values E and C were expressed as the concentration of IFN-gamma with ESAT-6 or CFP-10, minus the concentration of IFN-gamma in the nil control. Value E or C > or =0.35IU/mL was considered positive, > or =0.10 and<0.35IU/mL as equivocal (gray zone), and<0.10IU/mL as negative. We analyzed 8 patients with value E or C > or =0.10IU/mL at a preincubation time of 1 h. RESULTS: Value E and C decreased especially for preincubation time >6 h. As a result, the interpretation of value E changed from "positive" to "equivocal" in 2 cases and from "equivocal" to "negative" in 2 cases. Interpretation of value C also changed from "positive" to "equivocal" or "negative" in 2 cases and from "equivocal "to "negative" in 1 case. Even if the higher of value E or C were used for analysis, QFT results changed in half of patients when preincubation time was>6 h. CONCLUSION: Since QFT results in half of patients changed when preincubation time was>6 h, incubation of whole blood should start < or =6 h after blood drawing.
机译：背景：第二代量体-TB（QFT）的引入能够诊断具有高特异性和敏感性的分枝杆菌（TB）感染。该体外诊断测试使用2个TB特异性蛋白质（ESAT-6和CFP-10）来刺激肝素的全血中的细胞，并检测由血细胞产生的干扰素-γ（IFN-Gamma）。当QFT在医院之外的实验室完成时，可能需要几个小时来运输血液样本。我们研究了QFT结果与血液收集时间之间的关系（预孵育时间）。方法：从结核病嫌疑人绘制的肝素化全血液立即运送到实验室。我们开始用ESAT-6，CFP-10，促磷酸（植物粒菌蛋白）和1,3,6,9和12小时，孵育4个等分试样的血液，并在拉伸后1,3,6,9和12小时。孵育后，通过ELISA测定每个等离子体样品中IFN-γ的浓度，并且表达值E和C与ESAT-6或CFP-10表示IFN-γ的浓度，减去IFN-γ的浓度无控制。值E或C>或= 0.35IU / mL被认为是阳性的>或= 0.10和<0.35i / mL，为凸起（灰色区域），以及<0.10iu / ml为阴性。我们在预孵育时间为1小时，分析了8例价值E或C>或= 0.10iu / ml患者。结果：值e和c尤其在预孵育时间> 6小时下降。结果，对2例中的“阳性”变为“阳性”变为“阳性”，并在2例中从“等源”到“阴性”。价值C的解释也从“正面”变为2例中的“正面”或“否定”，并在1个案例中从“等离子”到“负”。即使价值E或C的较高用于分析，QFT结果也会在预孵育时间> 6小时时变为一半的患者。结论：由于QFT导致一半的患者在预孵育时间> 6小时时改变，血管后的全血的孵化应开始<或= 6小时。

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