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首页> 外文期刊>感染症学雑誌 >Isolation and serotyping of Vero toxin-producing Escherichia coli (VTEC) from pig
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Isolation and serotyping of Vero toxin-producing Escherichia coli (VTEC) from pig

机译：从猪中分离和血清毒素的大肠杆菌（VTEC）的分离和血清型

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As a part of basic studies to elucidate the source of infection of Verotoxin-producing Escherichia coli (VTEC) infectious disease, fresh feces were collected from pigs raised in Kanto District (A and B Prefectures) and Kyushu District (C and D Prefectures) between April and October in 2000, and isolation, serotyping, toxin typing, and drug sensitivity test of VTEC were performed. 1) Of 411 fecal samples tested, VTEC was isolated from 44 samples (10.7%), consisting of 12 of 112 samples (10.7%) from A Prefecture, nine of 100 samples (9.0%) from B Prefecture, 18 of 99 samples (18.2%) from C Prefecture, and five of 100 samples (5.0%) from D Prefecture. 2) Forty-five isolates were serotyped. Four isolates (8.9%) were typed as type 3, but the remaining 41 isolates (91.1%) could not be typed. The four typed isolates consisted of two O112ac:H- isolates and one each of O126:H- and O157:H7. 3) Toxin was typed in 45 isolates. Twenty-seven (60.0%) and 17 isolates (37.8%) produced VT 2 and VT1, respectively, and one isolate (2.2%) produced both VT1 and VT2. 4) Drug sensitivity tests of 45 isolates were performed. All 45 isolates (100%) were multidrug-resistant that were resistant to multiple drugs. Nineteen, nine, four, four, seven, one, and one isolates were resistant to five, six, two, three, four eight, and nine drugs, respectively. The above findings confirmed contamination in all districts, although the VTEC isolation rate varied among the sampling districts. Serotyping clarified the presence of O157:H7 and O112ac:H- that are detected in human VTEC infectious disease. The drug sensitivity tests clarified the presence of many multidrug-resistant strains.
机译：作为阐明产生术治疗患有毒素的大肠杆菌（VTEC）传染病的感染来源的基础研究的一部分，从康托区（A和B县）和九州区（C和D Profectures）之间的猪收集新鲜粪便2000年4月和10月，并进行了隔离，血清型，毒素打字和VTEC的药物敏感性试验。 1）411次粪便样品中检测，VTEC从44个样品（10.7％）中分离，由来自县的112个样品（10.7％）中的12个，来自B县的100个样品（9.0％），18个样品（ 18.2％）来自C Prefecture，来自D县的100个样品中的五种（5.0％）。 2）45个分离物血清液。四个分离株（8.9％）键入3型，但剩余的41分离株（91.1％）无法输入。四种类型的分离株由两种O112Ac：H-分离物和O126：H-和O157：H7中的一个分离物组成。 3）毒素在45个分离物中键入。二十七（60.0％）和17个分离株（37.8％）分别产生VT 2和VT1，一个分离物（2.2％）产生VT1和VT2。 4）进行45个分离物的药物敏感性试验。所有45个分离物（100％）是多药抗性，对多种药物耐药。十九，四，四，四，七，一个和一种分离物分别抵抗五个，六个，两种，三个，八个和九种药物。上述调查结果证实了所有地区的污染，尽管采样区的VTEC隔离率变化。血清型阐明了O157：H7和O112AC的存在：H-在人类vtec传染病中检测到。药物敏感性试验澄清了许多多药抗性菌株的存在。

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