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首页> 外文期刊>感染症学雑誌 >Evaluation of an improved pretreatment method for the measurement of (1-->3)-beta-D-glucan in blood samples
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Evaluation of an improved pretreatment method for the measurement of (1-->3)-beta-D-glucan in blood samples

机译：评价血液样品中（1 - > 3）-β-D-葡聚糖测量的改进预处理方法

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    Measurement of blood (1-->3)-beta-D-glucan is useful for early diagnosis and follow-up of the therapeutic process of deep seated mycoses. The Fungitec G test MK (Seikagaku Corp., Tokyo) kit using alkaline-pretreatment followed by chromogenic kinetic assay has been widely used in Japan because of its high sensitivity and easy handling of a large number of samples. Discrepancy in the levels of (1-->3)-beta-D-glucan and/or in the quantitative judgement, however, has been pointed out between this kit and other commercial kits. One of the reasons for this discrepancy has been reported to be non-specific reactions caused by substances other than beta-glucan. In this study, we have improved the alkaline pretreatment reagent by changing the concentration of KOH and salts, resulting in a marked reduction of the non-specific reaction. Recovery of standard beta-glucan added to plasma or serum after the improved pretreatment was 80 to 120%, and no amidolytic activity was detected either in plasma or in serum. By the improved pretreatment, the incidence of non-specific reactions, i.e., those that exceed the quantitation limit (3.9 pg/mL), were markedly decreased from 139 to 16 out of 200 plasma samples and from 106 to 22 out of 170 serum samples. The incidence of strong non-specific reactions, i.e., those that exceed the cut-off level (20 pg/mL), were also decreased from seven to one with plasma and seven to zero with serum samples. Correlation between corrected beta-glucan measurements by the current pretreatment and non-corrected ones by the improved pretreatment was quite good. The improved method is thus expected to decrease the frequency of non-specific false-positive reactions, with the high sensitivity of Fungitec G test MK.
    机译：血液（1 - > 3）-Beta-D-Glucan的测量可用于早期诊断和后续坐姿肌肉治疗过程的后续。使用碱性预处理的Fungitec G Test MK（Seikagaku Corp.，Tokyo）试剂盒，然后是发色动力学测定，由于其高灵敏度和易于处理大量样品，因此在日本广泛使用。然而，在该套件和其他商业套件之间指出（1 - > 3）-Beta-d-glucan和/或定量判断的水平差异。据报道，这种差异的原因之一是由β-葡聚糖以外的物质引起的非特异性反应。在这项研究中，通过改变KOH和盐的浓度，我们改善了碱性预处理试剂，导致非特异性反应的显着降低。在改善预处理后加入到血​​浆或血清中添加的标准β-葡聚糖的回收率为80％至120％，并且在血浆中或血清中没有检测到酰上酰胺活性。通过改善的预处理，非特异性反应的发生率，即超过定量限制（3.9pg / ml），从200个血浆样品中的139-10分钟显着降低，170个血清样品中的106-22中。强的非特异性反应的发生率，即超过截止水平（20pg / ml）的那些，也从血浆和血清样品中的血浆和7到零降低。通过改善预处理的预处理和未校正的预处理的矫正β-葡聚糖测量之间的相关性相当不错。因此，预期改进的方法会降低非特异性假反应的频率，具有稳定性G试验MK的高灵敏度。

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