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首页> 外文期刊>感染症学雑誌 >Bacteriological features of Mycobacterium haemophilum isolated from skin lesions in an immunodeficient patient
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Bacteriological features of Mycobacterium haemophilum isolated from skin lesions in an immunodeficient patient

机译：免疫缺陷患者皮肤病患者分离的分枝杆菌的细菌特征

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A 53-year-old, male patient presented with pain in the middle area of the back of his left foot. The painful area was associated with a reddish dome-shaped swelling of 24 by 18 mm which had ulcerated in the center part. Histopathologically, the cutaneous lesion consisted of an ulcer surrounded by abscess and granuloma and numerous acid-fast organisms were observed. Subsequently, the area just below the left inguinal area developed redness and swelling approaching the size of a quail egg. The patient responded favorably with rifampicin, levofloxacin, and minocycline therapy. The patient was immunodeficient, but negative for HIV-1 and HIV-2 antibodies and the etiology of his immunodeficient state is unclear. Skin tissues or pus were cultured at 37 degrees C on 2% Ogawa and BBL MGIT. Acid-fast organisms were recovered on MGIT within 4 to 12 days, while 2% Ogawa medium failed to recover acid-fast bacteria. Using growth from the positive MGIT tube as inoculum, MycoBroth, 7H9 broth, 7H11.2% Ogawa supplemented with or without iron complexes, and blood agar were inoculated and cultured at 30 and 37 degrees C. Growth at 30 and 37 degrees C was seen with MycoBroth, 7H9, hemin (60 microM) or ferric ammonium citrate (15 mg/ml) supplemented 7H11 and blood agar as well as 7H11 supplemented with factor X. Growth at 30 degrees C only was observed for ferric ammonium citrate supplemented 7H9 and 2% Ogawa. Generally, growth at 30 degrees C was better than that at 37 degrees C in all media. No growth at either temperature was observed with hemin or factor X supplemented 2% Ogawa. With respect to the biochemical characterization, the isolate was negative for niacin, nitrate reduction, urease, arylsulfatase, Tween 80 hydrolysis, catalase, 68 degrees C catalase, acid phosphatase, and tellurite reduction, while strongly positive for neutral red test. Sequencing of the 16S rRNA gene showed the isolate to be consistent with Mycobacterium haemophilum. Based on the composite characterization, the isolate was identified asM. haemophilum. This is the second case report of M. haemophilum infection in Japan in the literature.
机译：一个53岁的男性患者，左脚后面的中间区域呈现疼痛。疼痛的区域与24×18毫米的红圆顶形肿胀有关，该肿胀在中心部分溃疡。组织病理学上，皮肤病变由脓肿包围的溃疡和肉芽肿，观察到许多酸快的生物。随后，左侧腹股沟面积下方的区域发红和肿胀接近鹌鹑蛋的大小。患者对利福平，左氧氟沙星和米诺环素治疗有利良好的反应。患者是免疫缺陷的，但HIV-1和HIV-2抗体的阴性和阴性缺陷状态的病因尚不清楚。在2％O ogawa和BBL MGIT上以37℃培养皮肤组织或脓液。在4-12天内在MgIT上回收酸快的生物，而2％OGAWA培养基未能回收酸快细菌。使用从正粉刺管的生长作为接种物，霉菌，7h9肉汤，7h11.2％ogawa，补充有或不含铁复合物，并且在30和37℃下接种和培养血液琼脂30和37℃的生长用霉菌，7H9，血红素（60微米）或铁氨基铵（15mg / ml）补充了7H11和血液琼脂以及补充有因子X的7H11。仅观察到30摄氏度的生长，用于补充7H9和2的铁铵氨铵％ogawa。通常，30摄氏度的生长优于所有培养基中的37摄氏度。用血红蛋白或因子X补充2％OGAWA的血红素或因子x没有观察到任何温度的生长。关于生化表征，分离物对于烟酸，硝酸盐还原，脲酶，芳基硫酸盐酶，吐温80水解，过氧化氢酶，酸性磷酸酶和碲酸盐减少，而中性红色试验强度呈阴性。 16S rRNA基因的测序显示分离物与血硫硅杆菌的一致。基于复合表征，鉴定了分离物。血粒子。这是日本文学中日本M.血红米感染的第二个病例报告。

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