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首页> 外文期刊>感染症学雑誌 >Countermeasures against non-specific reactions in the measurement of beta-glucan in plasma by alkaline treatment, chromogenic automated kinetic assay
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Countermeasures against non-specific reactions in the measurement of beta-glucan in plasma by alkaline treatment, chromogenic automated kinetic assay

机译：通过碱性处理测量β-葡聚糖测量中的非特异性反应的对策，发色自动动力学测定

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We investigated the detection of non-specific reactions in the measurement of plasma (1-->3)-beta-D-glucan (beta-glucan) and countermeasures against them using alkaline treatment, chromogenic automated kinetic assay (alkaline-kinetic assay). In this study, we reexamined the values of beta-glucan using the alkaline-kinetic assay with and without laminaran oligosaccharides (LO) as a kind of beta-glucan that blocks the Limulus reaction. The materials for this study were 584 plasma samples in which beta-glucan had been measured. These were taken from 232 patients in Kawasaki Medical School Hospital between January 2002 and March 2002. Non-specific reactions were judged by a calculated value under a LO additive condition. Determination as to whether or not the each time course of the Limulus reaction was influenced by a non-specific reaction was also studied by applying a non-specific reaction index set up independently. Non-specific reactions were recognized in 51.9% of the samples (81/156). The amount of non-specific reaction was 9.9 pg/ml or less in major samples. On the other hand, when the cut off value of the index for detection of non-specific reactions was set at 0.5, the sensitivity was 88.9% and specificity was 73.7%. The positive and negative predictive values were 93.5% and 60.9% respectively. Non-specific reactions can be approximately distinguished by applying the non-specific reaction index. By so doing, unnecessary initiation of anti fugal therapy in response to non-specific reactions can be avoided. Further prospective and radical studies of non-specific reactions in the alkaline-kinetic assay are necessary.
机译：我们研究了使用碱性处理的血浆（1-> 3）-β-Beta-D-葡聚糖（Beta-glucan）测量的非特异性反应的检测，并使用碱性处理，发色自动动力学测定（碱性动力学测定）对抗它们。在这项研究中，我们用碱性动力学测定用碱性动力学测定来审视β-葡聚糖的值，并且没有层状寡糖（LO）作为嵌段抑制硫酸的β-葡聚糖。本研究的材料是584个等离子体样品，其中测量了β-葡聚糖。这些来自2002年1月至2002年3月在川崎医学院医院的232名患者中获取。在LO添加剂条件下通过计算值判断非特异性反应。通过施加独立设定的非特异性反应指数，还研究了稀肢反应的每个时间过程的测定方法是否受到非特异性反应的影响。在51.9％的样品（81/156）中公认出非特异性反应。主要样品中非特异性反应量为9.9pg / ml或更低。另一方面，当将非特异性反应检测指数的切断值设定为0.5时，敏感性为88.9％，特异性为73.7％。阳性和消极预测值分别为93.5％和60.9％。通过施加非特异性反应指数，可以大致区分非特异性反应。通过这样做，可以避免不必要对非特异性反应反应的抗逃亡者治疗的启动。需要进一步的碱性动力学测定中的非特异性反应的进一步前瞻性和激进研究。

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