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Transferability of vanA gene from vancomycin-resistant Enterococcus faecalis in the digestive tract of specific pathogen-free mice

机译:Vana基因的可转移性来自Vancomycin肠球菌粪便在特定病原体小鼠的消化道中

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摘要

We evaluated the transferability of vanA gene from vancomycin-resistant Enterococcus faecalis (VREF) to vancomycin-sensitive E. faecalis (VSEF) in vitro and in vivo. In vitro conjugal transfer experiment by filter mating, the vanA gene of VREF was transferable at the high frequency to VSEF and a mutant strain which cured vanA gene of VREF. In vivo studies in the digestive tract of specific pathogen-free mice pretreated with oral antibiotics, transconjugants were also detected from the feces of a mouse at the lower frequency. However, the colonization of transconjugants was transient. The vanA gene in the donor and the transconjugant strain was confirmed by using a polymerase chain reaction method. These results suggest that VSEF colonizing in the human digestive tract might be developed to VREF by transferring of the vanA gene.
机译:我们评估了Vana基因从Vancomycin的抗肠杆菌粪便(VREF)在体外和体内vsecin敏感的vancomycin敏感的大肠杆菌(Vsef)。 通过过滤配合体外蛋白转移试验,VREF的VANA基因可在高频到VSEF和突变菌株,其固化VREF的VANA基因。 在使用口服抗生素预处理的特定病原体小鼠的消化道中的体内研究中,还在较低频率下从小鼠的粪便中检测到经杂交分子。 然而,经延长的转运剂的定植是瞬态的。 通过使用聚合酶链式反应方法确认供体中的vana基因和转蛋白菌株。 这些结果表明,通过转移VANA基因,可以向VREF开发人体消化道中的vsef殖民化。

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