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Characterization of Two Mobilizable Plasmids Isolated from Enterobacter cloacae

机译：从肠杆菌肝硬化中分离的可变质粒的表征

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We isolated two plasmids, pS5lA and pS51B which were 5782 bp and 4854 bp in size, respectively, from the third generation cephalosporin-resistant E, cloacae suspected to express metallo-beta-lactamase, and analyzed their structures. These two plasmids encode RNA I/RNA II genes for replication origin, relaxase genes of mobABCD for plasmid transfer, and several open reading frames. According to the classification of mobilizable plasmids by gene organization of the relaxases, pS51A and pS51B belong to the ColE1 super-family of mobilizable plasmids, commonly detected in Enterobacteriaceae. The metallo-beta-lactamase gene was not identified in either pS51A or pS51B by homology search of the putative open reading frames. Open reading frames encoded in pS51A include E. coli protein L-like, E' coli heat shock protein-like, and E. coli plasmid replication initiation protein-like, and those encoded in pS51B include helix-turn-helix protein-like, E. coli plasmid replication initiation protein-like, and Salmonella replication initiation protein-like. These plasmids are stably maintained in one strain of E. cloacae, thus, the encoded gene functions may confer growth advantage to the host cell.
机译：从第三代头孢菌素抗性E分别分别分别分别分离了两种质粒，PS5LA和PS51B，分别为5782bp和4854bp的尺寸，Cloacae可疑表达金属β-内酰胺酶，并分析它们的结构。这两种质粒编码RNA I / RNA II基因用于复制来源，Mobabcd的弛豫酶基因，用于质粒转移，以及几个开放阅读框架。根据通过基因组织的可流动性质粒的分类，PS51A和PS51B属于COLE1超级可移动性质粒，通常在肠杆菌薄膜中检测到。通过对推定的开放阅读框架的同源搜索，未在PS51A或PS51B中识别金属β-内酰胺酶基因。在PS51a中编码的开放阅读帧包括大肠杆菌蛋白L样，E'COLI热休克蛋白样和大肠杆菌质粒复制起始蛋白样，并且在PS51B中编码的那些包括螺旋转螺旋蛋白样，大肠杆菌质粒复制起始蛋白样和沙门氏菌复制起始蛋白质。这些质粒稳定地保持在一种E.Cloacae中，因此，编码的基因函数可以赋予宿主细胞的生长优势。

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