Optimizing HPLC Sample Preparation

Caitlin Smith

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Optimizing HPLC Sample Preparation

机译：优化HPLC样品制备

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摘要

Careful sample preparation is important prior to high-performance liquid chro-matography (HPLC)/ultra high-performance liquid chromatogra-phy (UHPLC) analysis for at least three reasons. For one, sample concentration often is needed. Second, the sample may require buffer exchange and/or desalt-ing to place it in the appropriate solu-tion for liquid chromatography. Finally, each HPLC or UHPLC sample will need to undergo at least one filtration step to remove contaminants and particles prior to running it through the liquid chroma-tography column.

机译：出于至少三个原因，在进行高效液相色谱（HPLC）/超高效液相色谱（UHPLC）分析之前，精心准备样品非常重要。首先，通常需要样品浓度。其次，样品可能需要进行缓冲液交换和/或脱盐，以将其置于液相色谱的适当溶液中。最后，每个HPLC或UHPLC样品在通过液相色谱柱之前，都需要经历至少一个过滤步骤以除去污染物和颗粒。

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来源
《American laboratory》 |2012年第2期|共4页
作者
Caitlin Smith;
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原文格式 PDF
正文语种 eng
中图分类化学;
关键词
Sample; Preparation; concentration;

机译：样品;制备;浓度;

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Optimizing HPLC Sample Preparation

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