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首页> 外文期刊>農業機械学会誌 >Development of the method to measure the water permeability of cell membrane using two-laminar flows in microchannel
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Development of the method to measure the water permeability of cell membrane using two-laminar flows in microchannel

机译：使用微通道中使用双层流量测量细胞膜水渗透性的方法的发展

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摘要

Water permeability coefficient (L{sub}p) of cell membrane is related to the microscopic water migration in tissue of the fruits and vegetables, which has an effect on the quality of the fruits and vegetables. To measure the L{sub}p, extra-cellular solution should be replaced quickly to apply osmotic stress to cells. However, the conventional methods take a relatively long time for the replacement of solutions which leads to noticeable errors, otherwise they require a large number of samples. In this study, a new method named "two-laminar flows method" was developed, and the new method solved the problems inherent to conventional methods. Comparing with data on spinach leaf L{sub}p determined by micropipette method, one of the conventional method, and two-laminar flows method, it was confirmed that an accurate measurement was made by two-laminar flows method.
机译：细胞膜的水渗透系数（L {Sub} P）与水果和蔬菜组织中的微观水迁移有关，这对水果和蔬菜的质量产生了影响。 为了测量L {sub} P，应快速更换细胞溶液以将渗透胁迫施加到细胞。 然而，传统方法需要相对较长的时间来替换导致显着误差的溶液，否则它们需要大量样品。 在这项研究中，开发了一种名为“双层流动方法”的新方法，新方法解决了传统方法所固有的问题。 与菠菜叶片L {亚} P的数据进行比较，通过微液化电液法测定，传统方法和双层流动方法之一，证实了通过双层流动方法进行精确的测量。

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