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Low-voltage producing microbial fuel cell constructs using biofilm-forming marine bacteria

机译:使用生物膜形成海洋细菌的低压产生微生物燃料电池构建体

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摘要

Biofilm-forming marine bacterial isolates Paenibacillus lautus NE3B01, Pseudomonas mendocina NR802, Stenotrophomonas acidaminiphila NCW702 and Pseudomonas pseudoalcaligenes NP103 in microbial fuel cell (MFC) were investigated for low-voltage power generation. Biofilm formation by the isolates was evaluated by glass tube assay, microtitre plate assay and fluorescence microscopy. A dual chamber MFC of 2 litre capacity was constructed for low-voltage power generation and current output. Two chambers were internally connected by salt bridge and externally the circuit was connected with copper wires which were joined to the electrodes at the two ends and to the multimeter. Maximum current was generated when the salt bridge was constructed using 1 M KCl for all the four bacterial isolates. With Paenibacillus lautus NE3B01, a maximum voltage of 727.5 13.4 mV in 6 h with 7 g/1 of glucose as the sole source of carbon was recorded. However, Pseudomonas mendocina NR802 MFC was the most stable in terms of potential generation among all the isolates used Tor MFC studies. The experimental data for current and voltage showed that the biofilm-forming marine bacterial isolates are useful in MFC technology.
机译:研究生物膜形成海洋细菌分离株Paenibacillus Ne3b01,Pseudomonas mendocina NR802,Stenotrophomonas acidaminiphila NCW702和Pseudomonas伪甲醛NP103在微生物燃料电池(MFC)中进行低压发电。通过分离株的Biofilm形成通过玻璃管测定,微量滴定板测定和荧光显微镜评估。为低压发电和电流输出构建了2升容量的双室MFC。通过盐桥内部连接两个腔室,外部电路与铜线连接,铜线连接到两端的电极和多电表。当使用1M KCl为所有四种细菌分离株构建盐桥时产生最大电流。使用Paenibacillus Lautus Ne3b01,记录了6小时的最大电压为727.5 13.4 mV,以7g / 1的葡萄糖记录为唯一的碳源。然而,假单胞菌门CONCINA NR802 MFC在所有隔离的MFC研究中的潜在生成方面是最稳定的。电流和电压的实验数据显示,生物膜形成海洋细菌分离株可用于MFC技术。

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