On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor

Cao Jiaming; Yang Xinghua; Teng Pingping; Liu Zhihai; Yang Jun; Zhang Jianzhong; Zhang Yu; Luo Meng; Gao Danheng; Kong Depeng; Xia Siyuan; Zhao Enming; Yuan Libo

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On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor

机译：基于光纤表面等离子体谐振传感器的柱色谱分离在线动态检测

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In this design, we introduced a surface plasmon resonance (SPR) fiber-sensing probe into a column chromatography (CC) system to realize on-line dynamic detection in sample separation. The refractive index of the gel around the probe would be adjusted dynamically by the concentration change of the sample during CC separation. To demonstrate the separation and on-line detection process, bovine serum albumin (BSA) and riboflavin-5-phosphate sodium (FMN-Na) are chosen as the analytes in a Sephadex gel filtration chromatography system. The results show that the apparent reversible shift of the SPR spectrum can characterize the separation process. Specifically, the separated BSA with an outflow time of 8 min can cause a resonance wavelength shift of 15.5 nm, and the FMN-Na with an outflow time of 26 min can cause a shift of 8.4 nm. This on-line dynamic detection of SPR spectra has great potential to save time and simplify the analysis process compared to the com- plex thin layer chromatography detection steps in traditional manual CC. (C) 2019 Optical Society of America

机译：在这种设计中，我们将表面等离子体共振（SPR）光纤感测探针引入柱色谱（CC）系统中，以实现样品分离中的在线动态检测。探针周围的凝胶的折射率将通过在CC分离期间样品的浓度变化来动态调节。为了证明分离和在线检测过程，牛血清白蛋白（BSA）和核黄素-5-磷酸钠（FMN-NA）作为Sephadex凝胶过滤色谱系统中的分析物。结果表明，SPR光谱的表观可逆偏移可以表征分离过程。具体地，具有8分钟的流出时间的分离的BSA可以导致15.5nm的共振波长偏移，并且具有26分钟的流出时间的FMN-NA会导致8.4nm的偏移。与传统手册CC中的COL薄层色谱检测步骤相比，SPR光谱的这种在线动态检测具有很大的节省时间，并简化分析过程。（c）2019年光学学会

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来源
《Applied optics》 |2019年第21期|共6页
作者
Cao Jiaming; Yang Xinghua; Teng Pingping; Liu Zhihai; Yang Jun; Zhang Jianzhong; Zhang Yu; Luo Meng; Gao Danheng; Kong Depeng; Xia Siyuan; Zhao Enming; Yuan Libo;
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Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Chinese Acad Sci Xian Inst Opt &

Precis Mech State Key Lab Transient Opt &

Photon Xian 710119 Shaanxi Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

Harbin Engn Univ Coll Sci Key Lab In Fiber Integrated Opt Minist Educ Harbin 150001 Heilongjiang Peoples R China;

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On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor

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