Accessible quantitative phase imaging in confocal microscopy with sinusoidal-phase synthetic optical holography

Canales-Benavides Arturo; Zhuo Yue; Amitrano Andrea M.; Kim Minsoo; Hernandez-Aranda Raul I; Carney P. Scott; Schnell Martin

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Accessible quantitative phase imaging in confocal microscopy with sinusoidal-phase synthetic optical holography

机译：具有正弦相合成光学全面的共聚焦显微镜中的可访问定量相位成像

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We present a technically simple implementation of quantitative phase imaging in confocal microscopy based on synthetic optical holography with sinusoidal-phase reference waves. Using a Mirau interference objective and low-amplitude vertical sample vibration with a piezo-controlled stage, we record synthetic holograms on commercial confocal microscopes (Nikon, model: AIR; Zeiss: model: ISM-880), from which quantitative phase images are reconstructed. We demonstrate our technique by stain-free imaging of cervical (HeLa) and ovarian (ES-2) cancer cells and stem cell (mHAT9a) samples. Our technique has the potential to extend fluorescence imaging applications in confocal microscopy by providing label-free cell finding, monitoring cell morphology, as well as non-perturbing long-time observation of live cells based on quantitative phase contrast. (C) 2018 Optical Society of America

机译：我们在基于具有正弦相参考波的合成光学全息术基于合成光学全息术的共聚焦显微镜中的定量相成像的技术简单地实现。使用Mirau干涉目标和低振幅垂直样本振动，通过压电控制阶段，我们在商业共聚焦显微镜上记录合成全息图（尼康，型号：AIR; Zeiss：型号：ISM-880），从中重建定量相位图像。我们通过宫颈（HELA）和卵巢（ES-2）癌细胞和干细胞（MHAT9A）样品的无染色成像来证明我们的技术。我们的技术具有通过提供无标记的细胞发现，监测细胞形态，以及基于定量相位对比度的无扰动的长时间观察，延长共聚焦显微镜中的荧光成像应用。（c）2018年光学学会

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《Applied optics》 |2019年第5期|共10页
作者
Canales-Benavides Arturo; Zhuo Yue; Amitrano Andrea M.; Kim Minsoo; Hernandez-Aranda Raul I; Carney P. Scott; Schnell Martin;
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Univ Rochester Inst Opt 275 Hutchison Rd Rochester NY 14620 USA;

Univ Illinois Beckman Inst Adv Sci &

Technol Urbana IL 61801 USA;

Univ Rochester Med Ctr Dept Microbiol &

Immunol Box 609 601 Elmwood Ave Rochester NY 14642 USA;

Univ Rochester Med Ctr Dept Microbiol &

Immunol Box 609 601 Elmwood Ave Rochester NY 14642 USA;

Tecnol Monterrey Eugenio Garza Sada 2501 Monterrey 64849 NL Mexico;

Univ Rochester Inst Opt 275 Hutchison Rd Rochester NY 14620 USA;

Univ Illinois Beckman Inst Adv Sci &

Technol Urbana IL 61801 USA;

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正文语种 eng
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Accessible quantitative phase imaging in confocal microscopy with sinusoidal-phase synthetic optical holography

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