Schlieren two-photon microscopy for phase-contrast imaging

Xie Hao; Zhang Yuanlong; Kong Linjie; Xi Peng; Dai Qionghai

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Schlieren two-photon microscopy for phase-contrast imaging

机译：Schlieren双光子显微镜检查相位对比度成像

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While simultaneous phase-contrast and two-photon fluorescence imaging in microscopy can bring abundant biomedical information, it is difficult to retrieve phase information from conventional two-photon microscopes. To realize low-cost, in situ phase-contrast and two-photon fluorescence imaging, we propose Schlieren two-photon microscopy, a method that implements phase-contrast imaging on two-photon microscopes. This method involves spatially modulated fluorescence plates, which are made of two-photon fluorescence dyes or upconversion nanoparticles. We demonstrate that the fluorescence intensity fluctuation reflects the phase gradients of the specimen via theoretical analysis, simulations, and experiments. The proposed method is fully compatible with commercial two-photon microscopes, thus enabling widespread applications in live tissue imaging. (C) 2018 Optical Society of America

机译：虽然显微镜中同时相位对比度和两光晶荧光成像可以带来丰富的生物医学信息，但难以从传统的双光子显微镜中检索相位信息。为了实现低成本，原位相位对比度和双光子荧光成像，我们提出了Schlieren双光子显微镜，这是一种在双光子显微镜上实现相位对比度成像的方法。该方法涉及空间调制的荧光板，其由双光子荧光染料或上转换纳米颗粒制成。我们证明荧光强度波动通过理论分析，模拟和实验反映了样本的相位梯度。该方法与商业双光子显微镜完全兼容，因此在直播组织成像中实现了广泛的应用。（c）2018年光学学会

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来源
《Applied optics》 |2019年第5期|共6页
作者
Xie Hao; Zhang Yuanlong; Kong Linjie; Xi Peng; Dai Qionghai;
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作者单位

Tsinghua Univ Dept Automat Beijing Peoples R China;

Tsinghua Univ Dept Automat Beijing Peoples R China;

Tsinghua Univ Dept Precis Instrument State Key Lab Precis Measurement Technol &

Instru Beijing Peoples R China;

Peking Univ Dept Biomed Engn Beijing Peoples R China;

Tsinghua Univ Dept Automat Beijing Peoples R China;

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正文语种 eng
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Schlieren two-photon microscopy for phase-contrast imaging

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