Evaluation of a flow cytometry method to determine size and real refractive index distributions in natural marine particle populations

Agagliate Jacopo; Roettgers Ruediger; Twardowski Michael S.; McKee David

摘要

A flow cytometric (FC) method was developed to retrieve particle size distributions (PSDs) and real refractive index (nr) information in natural waters. Geometry and signal response of the sensors within the flow cytometer (CytoSense, CytoBuoy b.v., Netherlands) were characterized to form a scattering inversion model based on Mie theory. The procedure produced a mesh of diameter and nr isolines where each particle is assigned the diameter and nr values of the closest node, producing PSDs and particle real refractive index distributions. The method was validated using polystyrene bead standards of known diameter and polydisperse suspensions of oil with known nr, and subsequently applied to natural samples collected across a broad range of UK shelf seas. FC PSDs were compared with independent PSDs produced from data of two LISST-100X instruments (type B and type C). PSD slopes and features were found to be consistent between the FC and the two LISST-100X instruments, but LISST concentrations were found in disagreement with FC concentrations and with each other. FC nr values were found to agree with expected refractive index values of typical marine particle components across all samples considered. The determination of particle size and refractive index distributions enabled by the FC method has potential to facilitate identification of the contribution of individual subpopulations to the bulk inherent optical properties and biogeochemical properties of the particle population. (C) 2018 Optical Society of America.

机译：开发了流式细胞术（Fc）方法以在天然水域中检索粒度分布（PSD）和实际折射率（NR）信息。流式细胞仪（Cytosense，Cytobuoy B.V.，荷兰）内传感器的几何和信号响应的特征是基于MIE理论形成散射反转模型。该过程产生的直径和NR ISOLINE的网状物，其中每个粒子被分配最近节点的直径和NR值，产生PSD和粒子实折射率分布。使用已知直径的聚苯乙烯珠子标准物和具有已知NR的多分散悬浮液的聚苯乙烯珠子标准物验证该方法，随后应用于跨越广泛的英国货物海洋收集的天然样品。将FC PSD与由两个LISST-100x乐器（B型和C型）的数据产生的独立PSD进行比较。发现PSD斜坡和特征在Fc和两个LISST-100x仪器之间是一致的，但在不同的浓度与Fc浓度和彼此不同，发现了LISST浓度。发现FC NR值在考虑的所有样品中达成典型海洋颗粒组分的预期折射率值。通过Fc方法实现的粒度和折射率分布的测定具有促进鉴定单个亚离子对颗粒群体的散装固有光学性质和生物地球化学性质的贡献。（c）2018年光学学会。

Evaluation of a flow cytometry method to determine size and real refractive index distributions in natural marine particle populations

摘要

著录项

相关主题

期刊订阅