Differential interference contrast microscopy for cells using hard x-ray holography

Matsuda K.; Lopez J. C. A.; Rehman S.; Misawa M.; Suzuki Y.; Takeuchi A.; Yasumoto M.; Hibino K.; Roy M.; Hanayama R.; Ishii K.

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Differential interference contrast microscopy for cells using hard x-ray holography

机译：使用硬X射线全息间的细胞差异干扰对比度显微镜

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We propose a differential interference contrast method for cells using hard x-ray Gabor holography and knife-edge filtering in the spatial frequency domain, without relying on beam shearing. A phase object is holographically recorded and reconstructed by computer. Interference between the wavefronts of zeroth order weighted by e(j pi/2) in the positive frequency region produces a dark image. Similarly, interference between the wavefronts of the zeroth order weighted by e(j3 pi/2) in the negative frequency region produces a bright image. By adding these two intensity distributions, good quality phase-contrast images of 8-mu m-diameter polystyrene beads and human HeLa cells were obtained. (C) 2018 Optical Society of America

机译：我们提出了使用空间频域的硬X射线Gabor全息和刀刃滤波的细胞的差异干扰对比方法，而不依赖于光束剪切。阶段对象被充分记录和由计算机重建。在正频区域中的E（J PI / 2）加权的Zeroth顺序的波前之间的干扰产生暗图像。类似地，在负频率区域中的e（J3 pi / 2）的Zeroth顺序的波前之间的干扰产生明亮的图像。通过加入这两个强度分布，获得了8-mu m型聚苯乙烯珠粒和人HeLa细胞的良好质量相位对比图像。（c）2018年光学学会

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《Applied optics》 |2018年第17期|共7页
作者
Matsuda K.; Lopez J. C. A.; Rehman S.; Misawa M.; Suzuki Y.; Takeuchi A.; Yasumoto M.; Hibino K.; Roy M.; Hanayama R.; Ishii K.;
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Natl Inst Adv Ind Sci &

Technol 1-2-1 Namiki Tsukuba Ibaraki 3058564 Japan;

Natl Inst Astrophys Opt &

Elect Luis Enrique Erro 1 Puebla Mexico;

1A Ripon Rd Clarence Pk Adelaide SA 5034 Australia;

Natl Inst Adv Ind Sci &

Technol 1-2-1 Namiki Tsukuba Ibaraki 3058564 Japan;

Japan Synchrotron Radiat Res Inst Spring 8 Sayo Hyogo 6795198 Japan;

Japan Synchrotron Radiat Res Inst Spring 8 Sayo Hyogo 6795198 Japan;

Japan Synchrotron Radiat Res Inst Spring 8 Sayo Hyogo 6795198 Japan;

Natl Inst Adv Ind Sci &

Technol 1-2-1 Namiki Tsukuba Ibaraki 3058564 Japan;

Univ New South Wales Sch Optometry &

Vis Sci Sydney NSW 2052 Australia;

Grad Sch Creat New Photon Ind Nishi ku 1955-1 Kurematsu Shizuoka Shizuoka 4311202 Japan;

Grad Sch Creat New Photon Ind Nishi ku 1955-1 Kurematsu Shizuoka Shizuoka 4311202 Japan;

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Differential interference contrast microscopy for cells using hard x-ray holography

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