Automatic, high-accuracy image registration in confocal microscopy

Liu Jian; Li Yong; Wang Weibo; Wang Yuhang; Zhang He; Tan Jiubin

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Automatic, high-accuracy image registration in confocal microscopy

机译：共聚焦显微镜中的自动，高精度图像配准

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We proposed a high-accuracy image registration method of confocal microscopy for a large field of view and high resolution. The spatial information (edge information) and the entropy correlation coefficient have been both taken into account for higher accuracy of registration. The edge information is introduced to calculate the normalization correlation coefficient of the image. Then the normalization correlation coefficient and the entropy correlation coefficient of the original image have been used to improve the proposed similarity measures, the normalized mutual information with edge information (called EMI). Meanwhile, a parallel particle swarm optimization (pa-PSO) with the idea of conditional initialization and parallel cooperation is developed to speed up the convergence rate and further reduce the mismatch. Experiments verified that the registration accuracy can be up to 0.2 pixel and has better robustness to the noise. (C) 2017 Optical Society of America

机译：我们提出了一种高精度的共聚焦显微镜图像登记方法，用于大视野和高分辨率。空间信息（边缘信息）和熵相关系数都考虑到更高的注册精度。引入边缘信息以计算图像的归一化相关系数。然后，已经使用了原始图像的归一化相关系数和熵相关系数来改善所提出的相似度测量，具有边缘信息（称为EMI）的归一化相互信息。同时，开发了一个平行粒子群优化（PA-PSO），以加速收敛速度并进一步减少不匹配来加速升级初始化和平行协作。实验证实，注册精度可以高达0.2像素，对噪声具有更好的稳健性。（c）2017年光学学会

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来源
《Applied optics》 |2017年第32期|共7页
作者
Liu Jian; Li Yong; Wang Weibo; Wang Yuhang; Zhang He; Tan Jiubin;
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Harbin Inst Technol Inst Ultra Precis Optoelect Instrument Engn Harbin 150001 Heilongjiang Peoples R China;

Harbin Inst Technol Inst Ultra Precis Optoelect Instrument Engn Harbin 150001 Heilongjiang Peoples R China;

Harbin Inst Technol Inst Ultra Precis Optoelect Instrument Engn Harbin 150001 Heilongjiang Peoples R China;

Harbin Inst Technol Space Environm Simulat Res Infrastruct Harbin 150001 Heilongjiang Peoples R China;

Harbin Inst Technol Inst Ultra Precis Optoelect Instrument Engn Harbin 150001 Heilongjiang Peoples R China;

Harbin Inst Technol Inst Ultra Precis Optoelect Instrument Engn Harbin 150001 Heilongjiang Peoples R China;

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Automatic, high-accuracy image registration in confocal microscopy

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