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Green optical dissolved oxygen sensor based on a chlorophyll-zinc complex extracted from the plant Brassica oleracea L.

机译:基于植物Brassica Oleracea L.提取的叶绿素锌复合物的绿色光学溶解氧传感器。

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摘要

In this study, we present the development of a green and high-sensitivity optical dissolved oxygen (DO) sensor for measuring DO in water using spectrofluorimetry. For the development of the sensor element, an organometallic complex was synthesized from the "chlorophyll A" molecule, extracted from the plant Brassica oleracea L., with zinc insertion (Zn + 2) replacing the magnesium (Mg + 2). In the investigation, we evaluated the fluorescence suppression of the organometallic zinc complex for samples with different concentrations of DO. The complex has shown two absorption regions, 350-475 nm and 600-700 nm. We observed the fluorescence suppression of the complex, when excited at 440 nm and analyzed at 635 nm, by using the spinning oxygenation and air pump flow methods, respectively. The fluorescence suppression curves presented first-order decay with good correlation, resulting in R2 = 0.98282 and R2 = 0.83849 for the spinning and air pump flow methods, respectively. To validate the methodology, we developed a prototype of a bench sensor. For the relation between the fluorescence intensity and oxygenation time, we obtained a ratio curve with R2 = 0.9809. The methodology and prototype sensor developed in this work are presented as a new optical method for the measurement of DO. (C) 2017 Optical Society of America
机译:在这项研究中,我们介绍了使用光谱氟化物测量水中的绿色和高灵敏度光溶解氧(DO)传感器的开发。为了开发传感器元件,从“叶绿素A”分子合成有机金属络合物,从植物芸苔甘油木L中提取,用锌插入(Zn + 2)替换镁(Mg + 2)。在调查中,我们评估了具有不同浓度的样品的有机金属锌复合物的荧光抑制。该综合体显示了两个吸收区,350-475nm和600-700nm。我们观察到复合物的荧光抑制,当在440nm时激发并在635nm处分析,分别通过使用旋转氧合和空气泵流动方法。荧光抑制曲线具有良好相关性的一阶衰减,得到纺丝和空气泵流动方法的R2 = 0.98282和R2 = 0.83849。为了验证方法,我们开发了一个台式传感器的原型。对于荧光强度和氧合时间之间的关系,我们获得了R2 = 0.9809的比率曲线。在本工作中开发的方法和原型传感器作为一种用于测量的新光学方法。 (c)2017年光学学会

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  • 来源
    《Applied optics》 |2017年第36期|共6页
  • 作者单位

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

    Fed Inst Educ Sci &

    Technol Ceara Dept Telecommun Photon Labs Av 13 Maio 2081 Fortaleza Ceara Brazil;

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  • 正文语种 eng
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