Effect of tissue frozen on quantitative optical properties using optical coherence tomography

Li J. H.; Chen J.; Mu X. J.; Shao Q. L.; Zhou Y. Q.; Yan L. J.

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Effect of tissue frozen on quantitative optical properties using optical coherence tomography

机译：光学相干断层扫描的组织冷冻对定量光学性能的影响

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The purpose is to demonstrate the optical charactering concerning nasopharyngeal tissue of pig by fresh sections and frozen correlating sections with optical coherence tomography (OCT).After being imaged on a fresh specimen, samples are then stored in low temperature refrigerators (-80 degrees C) for one year for the second OCT measurement. The OCT structure of the epithelium, lamina propria, and the basement membrane are still resolvable; the median scattering coefficients and anisotropy factors fitting from OCT images based on the multiple scattering effects for epithelium are 27.6 mm(-1) [interquartile range (IQR) 23.6 to 29.3 mm(-1)] versus 22.5 mm(-1) (IQR 20.5 to 24.4 mm(-1)), 0.86 (IQR 0.81 to 0.9) versus 0.88 (IQR 0.87 to 0.9) for fresh and frozen tissue, respectively; and 10.2 mm(-1) (IQR 8.1 to 13.6 mm(-1)) versus 9.6 mm(-1) (IQR 8.1 to 13.8 mm(-1)), 0.96 (IQR 0.93 to 0.98) versus 0.92 (IQR 0.9 to 0.98) for lamina propria, respectively. The results show that the frozen storage method can be used for OCT research. (C) 2017 Optical Society of America

机译：目的是通过新鲜截面和冷冻相关部分来证明猪的鼻咽组织的光学表现，并用光学相干断层扫描（OCT）。在新鲜标本上成像，然后将样品储存在低温冰箱（-80℃）中。第二个OCT测量一年。上皮，椎板丙虫和基底膜的OCT结构仍然可解决;基于上皮的多个散射效果的OCT图像中位散射系数和各向异性因子是27.6mm（IQR）23.6至29.3mm（-1）的27.6mm（IQR）（-1）（IQR 20.5至24.4毫米（-1）），0.86（IQR 0.81至0.9）分别为新鲜和冷冻组织的0.88（IQR 0.87至0.9）;和10.2毫米（-1）（IQR 8.1至13.6 mm（-1））与9.6 mm（-1）（IQR 8.1至13.8 mm（-1）），0.96（IQR 0.93至0.98）与0.92（IQR 0.9至分别为薄层丙虫0.98）。结果表明，冷冻存储方法可用于OCT研究。（c）2017年光学学会

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《Applied optics》 |2017年第30期|共5页
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Li J. H.; Chen J.; Mu X. J.; Shao Q. L.; Zhou Y. Q.; Yan L. J.;
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Shenzhen Inst Informat Technol Sch Mech &

Elect Engn Shenzhen 518172 Peoples R China;

Shenzhen Second Peoples Hosp Dept Nephrol Shenzhen 518037 Peoples R China;

Shenzhen Inst Informat Technol Sch Mech &

Elect Engn Shenzhen 518172 Peoples R China;

Shenzhen Inst Informat Technol Sch Mech &

Elect Engn Shenzhen 518172 Peoples R China;

Shenzhen Inst Informat Technol Sch Mech &

Elect Engn Shenzhen 518172 Peoples R China;

Shenzhen Inst Informat Technol Sch Mech &

Elect Engn Shenzhen 518172 Peoples R China;

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Effect of tissue frozen on quantitative optical properties using optical coherence tomography

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