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首页> 外文期刊>Analytical chemistry >High-performance liquid chromatography linked to inductively coupledplasma mass spectrometry and orthogonal acceleration time-of-flight massspectrometry for the simultaneous detection and identification ofmetabolites of 2-bromo-4-trifluoromethyl-[C-1
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High-performance liquid chromatography linked to inductively coupledplasma mass spectrometry and orthogonal acceleration time-of-flight massspectrometry for the simultaneous detection and identification ofmetabolites of 2-bromo-4-trifluoromethyl-[C-1

机译：高效液相色谱-电感耦合等离子体质谱联用和正交加速飞行时间质谱联用，用于同时检测和鉴定2-溴-4-三氟甲基-[C-1]的代谢物

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摘要

The use of HPLC coupled to inductively coupled plasma mass spectrometry (ICPMS) and orthogonal acceleration time-of-flight (oa-TOF) for the profiling, identification, and quantification of metabolites in rat urine following the administration of 2-bromo-4-trifluoromethylacetanilide is described, The metabolites present in the sample were separated by reversed-phase gradient chromatography with W-diode array detection. The bulk of the eluent (90%) from the UV detector was directed to an ICPMS where bromine-containing metabolites were detected and quantified using ICPMS. The minor portion of the eluent (10%) was taken for oa-TOFMS for identification. By these means, the metabolites were identified as sulfate and glucuronide conjugates of a ring hydroxy-substituted metabolite, a N-sulfate, a N-hydroxylamine glucuronide, and N- and N-hydroxyglucuronides.
机译：使用HPLC联用电感耦合等离子体质谱（ICPMS）和正交加速飞行时间（oa-TOF）进行2溴4给药后大鼠尿液中代谢产物的概况分析，鉴定和定量描述了三氟甲基乙酰苯胺。样品中存在的代谢物通过反相梯度色谱和W二极管阵列检测进行分离。来自紫外线检测器的大部分洗脱液（90％）被引导至ICPMS，在其中检测并使用ICPMS对含溴代谢物进行定量。洗脱液的一小部分（10％）用于oa-TOFMS鉴定。通过这些手段，将代谢物鉴定为环羟基取代的代谢物，N-硫酸盐，N-羟胺葡糖醛酸化物以及N-和N-羟基葡糖醛酸化物的硫酸盐和葡糖醛酸化物结合物。

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