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首页> 外文期刊>Biochemical Genetics >Genotype-Dependent Gene Expression in Strawberry (Fragaria x ananassa) Plants Under High Temperature Stress
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Genotype-Dependent Gene Expression in Strawberry (Fragaria x ananassa) Plants Under High Temperature Stress

机译：高温胁迫下草莓（Fragaria x ananassa）植物中的基因型依赖性基因表达

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摘要

The differences in tolerance to high temperatures were investigated on the basis of gene expressions in two strawberry (Fragaria x ananassaDuch) cultivars which were previously determined as high temperature tolerant (Redlands Hope = R. Hope) and sensitive (Festival). Plants were exposed incrementally to 35, 40, 45, and finally 50 degrees C for 24 h. qRT-PCR analyses were carried out with 19 known sequences from the databases. Protein expression analyses were based on SDS-PAGE results, sequenced and then separated due to their isoelectric points. Expression levels were determined at 35, 40, and 45 degrees C. According to the results, tolerance of 'R. Hope' to high temperature stress can be explained with the coordination of Hsp70, Hsp90, and small heat shock proteins (sHsps) having a vital and supplementary role in stress response. Sensitive cultivar 'Festival' can respond to high temperatures only with the low molecular weight protein and transcripts that do not take a central role in high temperature stress response. Moreover, allergen gene expression triggered by high temperature were detected in both cultivars with different expression levels. The greater expression level in allergen genes observed in the sensitive cultivar 'Festival' under high temperature indicates that there is possibly a negative correlation between expression level in allergen genes and heat stress tolerance. Future studies addressing allergen gene expression under high temperature stress are required to confirm on these findings and to expand on the potential use as a molecular marker in breeding process for enhanced tolerance to high temperature.
机译：在前面被确定为高温耐受性（雷德兰希望= R.希望）和敏感（节日）的基础上，研究了对高温对高温的差异的差异。植物逐渐地暴露于35,40,45，最终50℃，24小时。用来自数据库的19个已知的序列进行QRT-PCR分析。蛋白质表达分析基于SDS-PAGE结果，测序，然后由于其等电点分离。表达水平在35,40和45摄氏度下测定。根据结果，耐受性的耐受性。希望“高温应激能够用HSP70，HSP90和小型热休克蛋白（SHSP）在应力反应中具有重要和补充作用的协调来解释。敏感的品种“节日”可以对高温蛋白质和转录物进行响应高温，在高温应激反应中不采取核心作用。此外，在具有不同表达水平的栽培品种中检测到高温引发的过敏原基因表达。在高温下在敏感品种“节日”中观察到的过敏原基因的更大表达水平表明过敏原基因和热应激耐受性的表达水平可能存在负相关。需要在高温胁迫下解决过敏原基因表达的未来研究是在这些发现中确认并扩张在育种过程中作为分子标记的潜在用途，以提高对高温的耐受性。

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